Total Antioxidant
Activity, and Hesperidin, Diosmin, Eriocitrin and
Quercetin Contents of Various Lemon Juices
Mannan Hajimahmoodi1*,
Ghazaleh Moghaddam1, Seyed Mohsen Mousavi1,
Naficeh Sadeghi1, Mohammad Reza Oveisi1
and Behrooz Jannat2
1Department of Drug and Food
Control, Faculty of Pharmacy, Tehran University of
Medical Sciences, 2Drug and Food Control
Laboratory, Research Center, Ministry of Health and
Medical Education, Tehran, Iran
*For correspondence:
Email:
hajimah@sina.tums.ac.ir;
Tel/Fax: +98-21- 66954714
Received: 12 June 2013
Revised accepted: 27 March
2014
Tropical Journal of Pharmaceutical Research, June
2014; 13(6): 951-956
Abstract
Purpose: To investigate 38 natural
and 62 branded lemon juices for their total antioxidant
activities and flavonoid content.
Methods: The ferric reducing
antioxidant power was applied to evaluation of
antioxidant. Additionally quercetin, eriocitrin, diosmin
and hesperidin contents were evaluated by high
performance liquid chromatography (HPLC).
Results: Total antioxidant activity
of the juices ranged from 91.38 ± 0.01 to 526.93 ± 0.12
µg/ml. The maximum contents of quercetin, hesperidin and
eriocitrin 14.83 ± 2.83, 104.84 ± 12.58 and 10.68 ± 2.41
µg/mL, respectively. The natural juices had diosmin
levels as high as 236.18 mL. The limit of detection (LOD)
ranged from 0.15 µg mg-1 for hesperidine to 0.53 µg ml-1
for diosmin while the limit of quantification (LOQ)
ranged from 0.27 µg mg-1 for hesperidine to 0.93 µg ml-1
for quercetin.
Conclusion: The developed method can
also be applied to the determination of related extracts
of orange cultivars as well as various extracts of
medicinal plants.
Keywords: Antioxidant, Flavonoid,
Hesperidin, Diosmin, Eriocitrin, Quercetin, Lemon juice
