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Research Article


 

Antioxidant and Antihaemolytic Activities of the Leaves of Kefe cumin (Laser trilobum L) Umbelliferae

 

Mohammad Ali Ebrahimzadeh1, Seyed Mohammad Nabavi1,2, Seyed Fazel Nabavi1, Bahman Eslami3, Zahra Rahmani4*

1Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran University of Medical Sciences, Sari, 2Young Researchers Club, Islamic Azad University, Ghaemshahr Branch, Ghaemshahr, 3Department of Biology, Islamic Azad University, Ghaemshahr branch, 4Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran.

 

*Corresponding author:  E-mail: zadeh20@yahoo.com  Tel: +98-151-3543081-3; Fax: +98-151-3543084

Received: 9 January 2010                                                                                                     Revised accepted: 23 July 2010

Tropical Journal of Pharmaceutical Research, October 2010; 9(5): 441-449

 

Abstract

 

Purpose: To investigate the antioxidant and antihaemolytic properties of the leaves of Laser trilubum grown in Gaduk, Iran.

Methods: The antioxidant and antihaemolytic activities of the hydroalcohol extract of L. trilobum L. leaf were investigated by haemoglobin-induced linoleic acid peroxidation, scavenging of 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, nitric oxide and hydrogen peroxide, as well as by assessment of reducing power and Fe2+ chelating activity, using standard methods. Phenol and flavonoid contents were determined as gallic acid and quercetin equivalents, respectively.

Results: The extract showed antioxidant activity in some models, with 50 % inhibitory concentration (IC50) for DPPH radical-scavenging activity being 147.1 ± 7.5 mg ml-1. The extract showed good nitric oxide-scavenging activity of between 0.1 and 1.6 mg ml-1 (IC50 = 517.7 ± 23.1 vs. 20 ± 0.01 µg ml-1 for quercetin), weak Fe2+ chelating ability (IC50, 906.9 ± 37.8 mg ml-1), and low antioxidant activity in haemoglobin-induced linoleic acid system. However, it was capable of scavenging hydrogen peroxide in a concentration-dependent manner while also exhibiting potent antihaemolytic activity against H2O2 - induced haemolysis (IC50, 169.6 ± 6.9 mg ml-1). Total phenolic content was 75 ± 3 mg gallic acid equivalent/g and total flavonoid content 59.2 ± 2.1 mg quercetin equivalent/g. The amounts of gallic acid, quercetin and rutin were 0.99 ± 0.04, 0.63 ± 0.01 and < 0.10 μg/mg, respectively.

Conclusion: L. trilobum exhibited good but varying levels of antioxidant and antihaemolytic activities in nearly all the models studied, when compared with controls.  

 

Keywords: Antioxidant activity, Laser trilobum, Antihaemolytic, Flavonoids, Kefe cumin

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