Prasit Suwannalert1 
,
Nasapon Povichit2,3,
Pranom Puchadapirom1,
Mutita Junking4
For correspondence:- Prasit Suwannalert Email: parasit109@yahoo.com Tel:+6622015578
Received: 26 June 2011 Accepted: 17 January 2012 Published: 21 February 2012
Citation: Suwannalert P, Povichit N, Puchadapirom P, Junking M. Anti-Aging Activity and Non-Toxic Dose of Phytooxyresveratrol from Artocarpus lakoocha Roxb. Trop J Pharm Res 2012; 11(1):69-74 doi: 10.4314/tjpr.v11i1.9
© 2012 The authors.
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Purpose: To determine the anti-aging activity and toxicity doses of phytooxyresveratrol extracted from Artocarpus lakoocha Roxb.
Methods: Artocarpus lakoocha 100 g was extracted with 2 ml of 95 % ethanol to obtain phytooxyresveratrol (POV). Total phenolic content, as well as free radical scavenging and anti-glycation activities of POV were characterized in order to assess its anti-aging properties. The models of DNA nicking and bacterial reverse mutation (Ames) were applied to the extract in order to determine its effective and toxic doses, respectively.
Results: Phytooxyresveratrol (POV) exhibited antiaging activity. It also showed high levels of phenolic content, radical scavenging activity and anti-glycation. A POV concentration of 25 µg/ml promoted strong anti-DNA nicking. Furthermore, it was non-toxic at concentrations ranging from 5 - 100 µg/ml.
Conclusion: In vitro, phytooxyresveratrol (POV) extracted from Artocarpus lakoocha exerted effective anti-aging activity at a concentration of 25 µg/ml. Thus, the substance should be further studied for possible formulation into pharmaceutical products.
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