Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

In vitro antidiabetic, anti-inflammatory and antioxidant potential of the ethanol extract of Uromastyx hardwickii skin

Waqas Ahmad Shams¹, Gauhar Rehman¹, Samuel Okwudili Onoja² , Abid Ali¹, Khurshaid Khan¹, Sadaf Niaz¹

¹Department of Zoology, Abdul Wali Khan University, Mardan, Pakistan; ²Department of Veterinary Physiology and Pharmacology, Michael Okpara University of Agriculture, Umudike PMB 7267, Umuahia, Abia State, Nigeria.

For correspondence:- Samuel Onoja Email: samonreal@yahoo.com

Accepted: 17 September 2019 Published: 31 October 2019

Citation: Shams WA, Rehman G, Onoja SO, Ali A, Khan K, Niaz S. In vitro antidiabetic, anti-inflammatory and antioxidant potential of the ethanol extract of Uromastyx hardwickii skin. Trop J Pharm Res 2019; 18(10):2109-2115 doi: 10.4314/tjpr.v18i10.16

© 2019 The authors.
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Abstract

Purpose: To evaluate the in vitro antidiabetic, anti-inflammatory and antioxidant potential of the ethanol extract of Uromastyx hardwickii Skin (UHSEE).

Methods: The in vitro effects of UHSEE at various concentrations (10 - 250 µg/mL) on the activities of x572;-amylase, x572;-glucosidase and glucose uptake by yeast cells were used to evaluate its antidiabetic potential. Nitric oxide (NO), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide inhibitory assay were employed to determine its antioxidant effects, while the anti-inflammatory effects were evaluated using human red blood cell (HRBC) membrane stabilization assay.

Results: UHSEE inhibited x572;-amylase and x572;-glucosidase enzymes but increased glucose uptake by yeast cells in a concentration-dependent manner (p < 0.05). It also inhibited NO, DPPH, hydrogen peroxide and HRBC hemolysis in a concentration-dependent manner (p < 0.05).

Conclusion: Uromastyx hardwickii skin exhibits promising good antidiabetic, antioxidant and anti-inflammatory properties in vitro. However, its true potentials in this regard needs to be evaluted in vivo

Keywords: Uromastyx hardwickii skin, Antidiabetic, Antioxidant, Anti-inflammatory, 2,2-Diphenyl-1-picrylhydrazyl, Membrane stabilization