Xiao-Kun Yang1,2,
Ya-Dong Yang1,
Shu-Qian Tang1,
Ling Xu1,
Gui-Hong Yang1,
Qiao-Yu Xu1,
Hui Tang1,
Jin-Jin Wu1 
For correspondence:- Jin-Jin Wu Email: wujjtmmu@126.com Tel:+8602368757599
Received: 17 April 2013 Accepted: 3July 2013 Published: 23 August 2013
Citation:
Yang X, Yang Y, Tang S, Xu L, Yang G, Xu Q, et al.
Inhibitory Effect of Polysaccharides from Scutellaria barbata D. Don on Invasion and Metastasis of 95-D Cells Lines via Regulation of C-MET and E-CAD ex
© 2013 The authors.
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Purpose: To investigate the inhibitory effect of polysaccharides from Scutellaria barbata (PSB) on invasion and metastasis of lung cancer, and study the possible mechanism.
Methods: PSB was extracted with water and by alcohol precipitation, and purified by DEAE-52 column chromatography. A highly invasive and metastatic lung carcinoma cell, 95-D cell line, was used for the study. Cell adhesion and invasion assays in vitro were performed to evaluate the anti-invasive and anti-metastatic effects of PSB (50 - 200 μg/ml) on 95-D cell. Immunocytochemical staining and Western blot techniques were employed to study the regulatory effects of PSB on the ex
Results: The results indicate that PSB significantly inhibited cell invasion and migration of 95-D in a concentration-dependent manner (p < 0.05). The adhesion of 95-D cells to fibronectin was also inhibited by PSB (p < 0.05). The ex
Conclusion: Treatment with PSB can significantly inhibit the invasion and metastasis of 95-D cells in vitro, probably through the regulation of C-MET and E-CAD.
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