Ahmed Al-Harrasi1 
,
Liaqat Ali1,
Eliezer Ceniviva2,
Ahmed Al-Rawahi1,
Javid Hussain1,
Hidayat Hussain1,
Najeeb ur Rehman1,
Ghulam Abbas1,3,
Rashid Al-Harrasi1
For correspondence:- Ahmed Al-Harrasi Email: aharrasi@unizwa.edu.om Tel:+96892828487
Received: 17 July 2012 Accepted: 4 June 2013 Published: 23 August 2013
Citation: Al-Harrasi A, Ali L, Ceniviva E, Al-Rawahi A, Hussain J, Hussain H, et al. Antiglycation and Antioxidant Activities and HPTLC Analysis of Boswellia sacra Oleogum Resin: The Sacred Frankincense. Trop J Pharm Res 2013; 12(4):597-602 doi: 10.4314/tjpr.v12i4.23
© 2013 The authors.
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Purpose: To evaluate antiglycation and antioxidant activities as undertake HPTLC analysis of Boswellia sacra resin.
Methods: Sub-fractionation of the crude methanol extract of Hougari regular (HR) grade resin of Boswellia sacra was carried out by vacuum liquid chromatography. Free radical scavenging and anti-glycation activities of sub-fractions were characterized in order to assess their anti-aging properties. Furthermore, high performance thin layer chromatography (HPTLC) analysis of Boswellia sacra resins was also carried out.
Results: Polar fractions of the extract obtained exhibited the highest antiglycation activity while non-polar fractions showed more than 50 % inhibition in superoxide anion scavenging assay. Scavenging activity of reactive oxygen species results indicate that non-polar sub-fractions showed > 50 % inhibition, except Shabi frankincense (SF) oil which showed 33 % inhibition. Dichloromethane (CH2Cl2) fraction, 40 % dichloromethane (CH2Cl2)/n-hexane sub-fraction, and SF oil showed moderate activity in di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) assay. Furthermore, HPTLC analysis indicates the presence of 11-keto-β-boswellic acid (KBA) and 3-O-acetyl-11-keto-β-boswellic acid (AKBA) along with some other terpenoids.
Conclusion: Various sub-fractions of Boswellia sacra exert effective antiglycation and antioxidant activities. The extracts should be studied further for possible formulation into pharmaceutical products.
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