William Arputha Sundar1 
,
Hariharan Nellaiah2
For correspondence:- William Sundar Email: williamas@rediffmail.com Tel:+919600338289
Received: 25 June 2013 Accepted: 25 June 2013 Published: 18 October 2013
Citation: Sundar WA, Nellaiah H. Production of Methioninase from Serratia Marcescens Isolated from Soil and its Anti-Cancer Activity against Dalton's Lymphoma Ascitic (DLA) and Ehrlich Ascitic Carcinoma (EAC) in Swiss Albino Mice. Trop J Pharm Res 2013; 12(5):699-704 doi: 10.4314/tjpr.v12i5.6
© 2013 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To evaluate the anticancer activity of an enzyme, methioninase, obtained from a soil isolate, Serratia marcesens, against Dalton’s lymphoma ascitic (DLA) and Ehrlich ascitic carcinoma (EAC) in Swiss albino mice.
Methods: Cancer was induced using DLA and EAC cells in Swiss albino mice by intraperitoneal injection. The mice were then treated with 10 and 20 mg/kg body weight of methioninase; 5-fluorouracil (5-FU) was used as standard.
Results: The results showed good improvement in haematological parameters, improvement serum enzymes and lipid profile, as well as increase in life span by up to 77 %, increase in body weight (p < 0.01), decrease in cancer cell count from (2.70 ± 0.40) × 106 to (1.86 ± 0.30) × 106 cells/ml compared to (2.70 ± 0.40) × 106 to (1.42 ± 0.25) x 106 cells/ml for the group treated with 5-FU. Solid tumor volume decreased from 5.82 ± 0.15 to 4.10 ± 0.11 ml as as against a decrease from 5.82 ± 0.40 to 3.56 ± 0.28 for the group treated with 5-FU.
Conclusion: The isolated enzyme, methioninase, has a good anti-cancer activity and needs to be further be investigated for this activity.
Fulltext in PDF