Baatartsogt Oyungerel1,
Seungyun Chung1,
Do-Young Yoon2,
Tae-Young Han3,
Il-Young Han4,
Kee-Tae Kweon5,
Kyeng-Min Kim6,
Gwang-Joo Jeon6,
Kang-Duk Choi6 
For correspondence:- Kang-Duk Choi Email: kchoi04@hknu.ac.kr Tel:+82316705422
Received: 25 December 2014 Accepted: 10 May 2015 Published: 29 June 2015
Citation: Oyungerel B, Chung S, Yoon D, Han T, Han I, Kweon K, et al. C5 extract induces apoptosis in B16F10 murine melanoma cells through extrinsic and intrinsic apoptotic pathways and sub-G1 phase arrest. Trop J Pharm Res 2015; 14(6):967-976 doi: 10.4314/tjpr.v14i6.5
© 2015 The authors.
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Purpose: To investigate the anti-cancer activities of C5 extract (C5E), a new herbal preparation from Korea, on B16F10 cells.
Methods: The anti-proliferative effects of C5E were assessed by culturing B16F10 cells in the presence or absence of C5E. Cell cycle progression was analyzed by PI staining using flow cytometry. The quantities of apoptosis-inducing proteins were measured by Western blot.
Results: C5E inhibited the proliferation of B16F10 cells but not human keratinocytes. C5E induced S phase arrest by interfering with cell regulatory factors such as cyclins B1, D1, D3, and E, and cyclin-dependent kinase 2, in B16F10 cells. Furthermore, immunoblot analysis confirmed that treatment with C5E induced apoptosis and cleaved caspase-3, poly (ADP-ribose) polymerase, via extrinsic pathway, whereas Bcl-2 ex
Conclusion: C5E shows promising anti-cancer activity and can be a useful adjuvant with vinblastine in combination therapeutic treatment of skin cancer.
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