Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

expression of MiR-9 promotes proliferation, migration and differentiation of human neural stem cells

Fei Zeng, Jing Xiong, Wei Ke, Jie Pu, Yanqiang Zhan

Department of Neurology, Renmin Hospital of Wuhan University, Wuhan 430060, China;

For correspondence:- Yanqiang Zhan Email: FrischMbro@yahoo.com Tel:+862788041911

Accepted: 24 January 2018 Published: 28 February 2018

Citation: Zeng F, Xiong J, Ke W, Pu J, Zhan Y. expression of MiR-9 promotes proliferation, migration and differentiation of human neural stem cells. Trop J Pharm Res 2018; 17(2):213-217 doi: 10.4314/tjpr.v17i2.3

© 2018 The authors.
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Abstract

Purpose: To investigate the effect of miR-9 on the proliferation, differentiation and migration of human neural stem cells (NSCs).

Methods: The expression of miR-9 was investigated by quantitative real-time polymerase chain reaction (RT-PCR). Cell proliferation was assessed by cell counting kit-8 (CCK8) assay, while cell migration was studied by Transwell assay. The effect of miR-9 on differentiation of NSCs was investigated by western blot analysis of key differentiation marker proteins. Protein expression was determined by western blotting.

Results: Transfection and over-expression of miR-9 in NSCs significantly enhanced the proliferation of NSCs (p < 0.05) in a time-dependent manner, as was evident from CCK8 assay data. MiR-9 over-expression caused down-regulation of Nestin and SOX-2, and up-regulation of Tuj-1 and MAP-2. The migration of NSCs was 37 % in the cells transfected with empty vector, compared to 68 % in the cells transfected with miR-9. This effect of miR-9 on cell migration was accompanied by up-regulation of matrix metallopeptidase 9 (MMP-9) and matrix metallopeptidase 2 (MMP-2).

Conclusion: These results show that miR-9 promotes the proliferation, differentiation and migration of NSCs, and thus may be an important drug target for the generation of NSCs

Keywords: Neural stem cells, MicroRNA, Mir-9, Migration, Differentiation, Proliferation