Imran Altaf1,
Faryal Ashraf1,
Muhammad Ashraf2,
Moneeb Ashraf3,
Aqeel Javeed2,
Neelma Munir1,
Rasheeda Bashir4 
For correspondence:- Rasheeda Bashir Email: rashidasbs@yahoo.com Tel:+923024680187
Accepted: 16 September 2020 Published: 310October 2020
Citation: Altaf I, Ashraf F, Ashraf M, Ashraf M, Javeed A, Munir N, et al. Evaluation of the cytotoxic and antiviral effects of ethanol extract of three Opuntia species of Peste des Petits ruminant virus. Trop J Pharm Res 2020; 19(10):2123-2128 doi: 10.4314/tjpr.v19i10.16
© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..
Purpose: To assess in vitro the virocidal effects of different species of cactus plant on the lethal action of Peste des petits virus (PPRV).
Method: Ethanol extracts of different cactus plants were obtained. A serial twofold dilution of the extracts was prepared. Cytotoxic and antiviral activities were examined through MTT assay at various concentrations. Vero cell lines were grown in 96 well plates up to an 80 % confluent monolayer. The plates were divided into two groups, one for antiviral and the other for cytotoxicity activity. The cells were exposed to various concentrations of the ethanol extracts to assess the cytotoxicity, whereas to assess the antiviral activity, PPRV was re-incubated with the extracts and then exposed to cells. MMT dye was added and the results were evaluated as cell survival (%).
Results: At higher concentrations, i.e., 500 - 1000 µg/mL, ethanol extracts from all the Opuntia species displayed cytotoxic effects. The ethanol extract of OM exhibited the greatest antiviral potential of all the extracts, while the extract of Opuntia stricta (OS) was the least effective against PPRV in the cultured cells. There were significant differences (p < 0.05) in the concentration of Opuntia manocantha (OM), Opuntia delinii OD and Opuntia stricta (OS) with reference to antiviral activity. OM showed antiviral activity against PPRV from 3.25 to 125 µg/mL, OD antiviral activity from 31.25 to 62.5 ug/ml whereas OS showed antiviral activity at 2.5 µg/mL
Conclusion: The ethanol extract of Opuntia species reduces the infection of PPRV in Caprine.
Archives
News Updates
Fulltext in PDF