Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

C1q/tumor necrosis factor (TNF)-associated protein 6 (CTRP6) ameliorates the cognitive dysfunction induced by sevoflurane by activating AMPK/SIRT1 pathway in rats

Yunming Nie¹, Yanyue Wu², Xuefen Huang¹, Zhengzheng Chen¹, Yimin Zhou³

¹Department of Anesthesia, Zhejiang Taizhou Hospital of Traditional Chinese Medicine, Taizhou, Zhejiang Province 318000; ²Department of Ophthalmology, Eye Hospital of Taizhou Municipal Hospital, Taizhou, Zhejiang Province 318000; ³Department of Anesthesiology, Wenzhou Hospital of Traditional Chinese Medicine, Wenzhou, Zhejiang Province 325000, China.

For correspondence:- Yimin Zhou Email: zhouyimin10826@163.com Tel:+8657756671319

Accepted: 17 February 2022 Published: 31 March 2022

Citation: Nie Y, Wu Y, Huang X, Chen Z, Zhou Y. C1q/tumor necrosis factor (TNF)-associated protein 6 (CTRP6) ameliorates the cognitive dysfunction induced by sevoflurane by activating AMPK/SIRT1 pathway in rats. Trop J Pharm Res 2022; 21(3):549-554 doi: 10.4314/tjpr.v21i3.14

© 2022 The authors.
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Abstract

Purpose: To evaluate the possible effects of C1q/tumor necrosis factor (TNF)-associated protein 6 (CTRP6) on postoperative cognitive dysfunction (POCD), including the potentially-related signaling pathway.

Methods: Behavioral analysis and cognitive impairment were assessed in each group. Immunoblots were used to determine the level of CTRP6 following sevoflurane-induced nerve injury. Hippocampal neurons were identified using Nissl staining, while inflammatory response following neuronal injury was monitored by enzyme-linked immunosorbent assay (ELISA) and quantitative polymerase chain reaction (qPCR). The involvement of adenosine monophosphate-activated protein kinase (AMPK)/sirtuin 1 (SIRT1) pathway was determined using immunoblot.

Results: CTRP6 alleviated sevoflurane-induced cognitive dysfunction in rats (p < 0.001). Moreover, CTRP6 reduced sevoflurane-induced nerve injury and inflammation in rats (p < 0.05).

Conclusion: CTRP6 ameliorates sevoflurane-induced cognitive dysfunction by activating AMPK/SIRT1 pathway, thus offering a novel target for POCD treatment.

Keywords: Postoperative cognitive dysfunction (POCD), C1q/Tumor necrosis factor (TNF) associated protein 6 (CTRP6), Nerve injury, Inflammation, AMPK/SIRT1 pathw