Dwi Wahyu Indrawati^{1, 2} , Ernie Maduratna Setiawatie³, Retno Pudji Rahayu⁴, Rini Devijanti Ridwan⁵, Hendrik Setia Budi⁵, Coen Pramono⁶, Ida Bagus Narmada^{7, 8}, Anita Yuliati⁹, Devi Riant¹⁰, Hari Basuki Notobroto¹¹, Rizky Briliant Syah Manurung¹²

Abstract

Purpose: To evaluate bovine pericardium membranes modified with 120 kDa hyaluronic acid (HA) for their physicochemical and biofunctional properties. Methods: Bovine pericardium membranes were soaked in different concentrations of HA solutions (0.5, 1.0 and 2.0 %) and analyzed using Fourier Transform Infrared Spectroscopy (FTIR) to identify functional groups, X-ray Diffraction (XRD) to assess crystallinity, and Scanning Electron Microscopy (SEM) combined with Energy-Dispersive X-ray Spectroscopy (EDS) was used to examine surface morphology and carry out elemental composition analysis. Results: The FTIR analysis revealed a stable collagen structure with preserved amide peaks and hydroxyl groups, indicating biochemical integrity. In contrast, the X-ray diffraction (XRD) results showed that all groups exhibited amorphous structures, suggesting flexibility suitable for soft tissue regeneration. Scanning electron microscopy showed a uniform morphology, while elemental analysis indicated that carbon (C), nitrogen (N), and oxygen (O) were the major elements present, with oxygen content increasing at higher HA concentrations, reflecting improved hydrophilic properties. Conclusion: Bovine pericardium membranes modified with 120 kDa HA exhibit preserved structural integrity, increased hydrophilicity, and uniform surface morphology, indicating their overall potential as scaffolds for wound repair and soft tissue engineering. Further optimization is necessary to improve degradation resistance and crystallinity for wider biomedical applications.