Fatima Talib Abdullah , Dhuha A Al Shammaa

Department of Pharmacognosy, College of Pharmacy, University of Baghdad, Baghdad, Iraq;

For correspondence:-  Fatima Abdullah   Email: ftalib248@gmail.com

Revised: 29 April 2026        Accepted: 17 June 2026        Published: 29 June 2026

Citation: Abdullah FT, Al Shammaa DA. In vitro assessment of the antiproliferative effects of Iraqi Euryops pectinatus ethyl acetate extract on MDA-MB-231 breast cancer cells. Trop J Pharm Res 2026; 25(6):797-802 doi: https://dx.doi.org/10.4314/tjpr.v25i6.5

© 2026 The authors.
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Abstract

Purpose: To investigate the antiproliferative activity of ethyl acetate extract of Iraqi Euryops pectinatus on MDA-MB-231 human breast cancer cells. Methods: The dried plant material was extracted by maceration with solvents of increasing polarity. The ethyl acetate extract was subjected to RP-HPLC for phytochemical profiling. The antiproliferative activity of the extract was assessed by MTT assay on MDA-MB-231 breast cancer cells. Results: Several phenolic and flavonoid compounds such as quercetin, apigenin, rutin, rosmarinic acid, caffeic acid and naringenin were present. Cytotoxic activity of the ethyl acetate extract was concentration-dependent against MDA-MB-231 cells with an IC?? value of approximately 2023 μg/mL. Cell viability decreased significantly with increasing extract concentrations, indicating moderate antiproliferative activity (p < 0.05). Observed activity may be associated with combined or synergistic effects of the identified phenolic and flavonoid constituents. Conclusion: Euryops pectinatus significantly exhibits cytotoxic activity against MDA-MB-231 breast cancer cells in a concentration-dependent manner, attributed to the synergistic effects of the phytoconstituents.

Keywords: Breast cancer, Cytotoxicity, Ethyl acetate extract, Euryops pectinatus, MDA-MB-231

Impact Factor
Thompson Reuters (ISI): 0.6 (2023)
H-5 index (Google Scholar): 49 (2023)