Kaiqi Ma,
Fujun Jin,
Qiaoli Wang,
Zhe Ren,
Kai Zheng,
Yifei Wang 
For correspondence:- Yifei Wang Email: twang-yf@163.com Tel:+862085223426
Received: 5 July 2014 Accepted: 20 February 2015 Published: 31 March 2015
Citation: Ma K, Jin F, Wang Q, Ren Z, Zheng K, Wang Y. siRNAs targeting viral protein 5: The major capsid protein of herpes simplex virus-1 affects its propagation and cytoskeleton. Trop J Pharm Res 2015; 14(3):391-397 doi: 10.4314/tjpr.v14i3.6
© 2015 The authors.
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Purpose: To investigate whether siRNA targeting viral protein 5 (VP5) can become a new treatment for herpes simplex virus type 1 (HSV-1).
Methods: Flow cytometry was performed to determine the ratio of siRNA and lipo2000 to reach the highest transfection efficiency. Western blot and q-PCR were performed to determine the knockdown efficiency of siRNA targeting UL19, as well as changes in cytoskeleton proteins. Plaque reduction assays and confocal microscopy were conducted to test the influence of VP5 knockdown on the HSV-1 life cycle and viral replication. F-actin organization was observed through confocal microscopy during HSV-1 infection when transfected with siRNA.
Results: Relative ex
Conclusion: Interfering with UL19 gene ex
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