Controlled Release Formulation
of Indomethacin Prepared With Bee Glue Extracts
S Honary1*,
P Ebrahimi2, F Naghibi3, M
Chaigani1
1Mazandaran
University of Medical Sciences, School of Pharmacy,
Pharmaceutical Sciences Research Center, Sari,
2Gonbad
Institute of Higher Education Center, P.O. Box 163,
Gonbad, 3 Traditional Medicine & Materia
Medica Research Center (TMRC) Shaheed Beheshti
University of Medical Sciences, Tehran, Iran
For correspondence:
E-mail:
shonary@mazums0ac.ir,
shonary@yahoo.com
Tel: 0098 912 145 2220
Received: 27
December 2010 Revised
accepted: 30
July 2011
Tropical
Journal of Pharmaceutical Research, Oct 2011;
10(5): 543-550
http://dx.doi.org/10.4314/tjpr.v10i5.2
Abstract
Purpose:
To prepare and evaluate new sustained release
formulations of indomethacin based on extracts of
propolis (bee glue).
Methods:
Standardization of propolis (bee glue) extracts was
performed by high performance liquid chromatography (HPLC)
and determination of the values of fat and fixed oils.
Several indomethacin capsule formulations (F1 - F18)
containing varying amounts of chloroform (0.75 - 75 mg)
and ethanol extracts (30 - 75 mg) of propolis were
prepared. The dissolution rate of the formulations was
evaluated by USP dissolution (rotating basket) method I
and the release data subjected to various kinetic
models. Probable interaction between the drug and
propolis extracts was studied by differential scanning
calorimetry (DSC).
Results:
The results show that, although the release rate of
formulations F1 - F7 did not show any significant
difference (p < 0.05) compared to F18 as blank, the
other formulations did. DSC results indicate that
incorporation of propolis extract in the formulations
lowered indomethacin melting point by between 5 and 30
ºC, indicating interaction between the drug and the waxy
extract. Kinetic analysis of the in vitro release data
of the formulations showed that the best-fit drug
release model varied with the drug:propolis extract
ratio of the formulations.
Conclusion:
Formulation F13 (with equal proportion of drug and bee
glue extract) came out best from the dissolution test
for indomethacin extended-release capsules as it
exhibited zero order kinetics. This fpormulation is
therefore suitable for further development as a matrix
formulation for controlled release.
Keywords:
Propolis (bee glue),
Indomethacin, Controlled release, Zero order kinetics,
Waxy materials
