Development and Application of Ligand-Exchange
Reaction Method for the Determination of Clonazepam
Snežana S Mitić,
Aleksandra N Pavlović*,
Snežana B Tošić, Emilija T Pecev, Milan N Mitić and
Milan B Stojković
University of Niš, Faculty of Sciences and Mathematics,
Department of Chemistry,
Višegradska 33, P.O.Box 224,
18000 Niš, Serbia
*For correspondence:
Email:
pavlovic_an@yahoo.com;
Tel: +381 18 533-015; Fax: +381 18 533-014
Received: 16 April
2011 Revised
accepted: 9 December 2011
Tropical Journal of
Pharmaceutical Research, February 2012; 11(1):
91-98
http://dx.doi.org/10.4314/tjpr.v11i1.12
Abstract
Purpose:
This paper presents an improved kinetic-spectrophotometric
procedure for determining clonazepam (CZP) in
pharmaceutical formulations and human serum.
Methods:
The method is based on
ligand-exchange
reaction. The
reaction was followed spectrophotometrically by
measuring the rate of change of absorbance at 425 nm in
ethanolic sodium hydroxide solution.
Results:
The optimum operating conditions for reagent
concentrations and temperature were established. Linear
calibration curve was obtained in the range of 0.32 -
4.10 µg mL-1. The optimized conditions
yielded a theoretical detection limit of 0.24 µg mL-1
based on the 3.3So criterion, where S0
is standard deviation of the calibration line. The
interference of certain drugs, foreign ions and amino
acids on the reaction rate were studied in order to
assess the selectivity of the method.
Conclusion:
The developed method is sensitive, accurate and
reproducible and
could be used
for routine anlysis of
clonazepam
in pharmaceutical preparations and serum samples.
Keywords:
Clonazepam, Ligand-exchange reaction, Kinetic
spectrometry, Validation, Pharmaceutical preparation,
Human serum.
