Purpose: This study aimed to determine the frequency rates of human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), and syphilis among blood donors.

Methods: Physically fit persons aged 18 – 48 years who came for blood donation at the blood bank unit of the military hospital in Hodeidah, Yemen  (MHH) from November 2008 to October 2010 were screened using standard diagnostic (SD) reagents. Based on the results, donors with clinical anemia and with history of jaundice were excluded.

Results: A total of 1,483 male donors (96 % semi-voluntary and 4 % replacement donors) with a mean age of 24.3 years were enrolled in this study. The frequencies of HBV, HCV, HIV and syphilis in the samples were 2.35, 0.79, 0.14, and 0.34 %, respectively. Compared with the first year, the decrease in HBV and HCV positive cases and the increase in HIV and syphilis positive cases in the second year were not statistically significant (p = 0.91, p = 0.74, p = 0.72, and p = 0.92, respectively).

Conclusion: While the frequency rate of transfusion-transmitted infections (TTIs) is low, it remains a major problem in blood transfusion. Proper protocol should be applied in selecting and screening donors to safeguard the health of people receiving blood transfusions.