Anti-Aging Activity and Non-Toxic Dose of
Phytooxyresveratrol from Artocarpus lakoocha Roxb
Prasit Suwannalert1*,
Nasapon Povichit2,3, Pranom Puchadapirom1
and Mutita Junking4
1Department
of Pathobiology, Faculty of Science, Mahidol University,
Bangkok 10400, 2Detox (Thailand) Co. Ltd.,
Chiang Mai 50200, 3Youcando R&D Institute,
Youcando Co Ltd., Bangkok 10510, 4Division
of Medical Molecular Biology, Department of Research and
Development, Faculty of Medicine Siriraj Hospital,
Mahidol University, Bangkok 10700, Thailand
*For correspondence:
Email:
parasit109@yahoo.com,
scpsw@mahidol.ac.th
Tel: +66-2-201-5578; Fax:
+66-2-354-715,
Received: 26 June
2011 Revised
accepted: January 17 2012
Tropical Journal of
Pharmaceutical Research, February 2012; 11(1):
69-74
http://dx.doi.org/10.4314/tjpr.v11i1.9
Abstract
Purpose:
To determine the anti-aging activity and toxicity doses
of phytooxyresveratrol extracted from Artocarpus
lakoocha Roxb.
Methods:
Artocarpus lakoocha 100 g was extracted with 2 ml of 95
% ethanol to obtain phytooxyresveratrol (POV). Total
phenolic content, as well as free radical scavenging and
anti-glycation activities of POV were characterized in
order to assess its anti-aging properties. The models of
DNA nicking and bacterial reverse mutation (Ames) were
applied to the extract in order to determine its
effective and toxic doses, respectively.
Results:
Phytooxyresveratrol (POV) exhibited antiaging activity.
It also showed high levels of phenolic content, radical
scavenging activity and anti-glycation. A POV
concentration of 25 µg/ml promoted strong anti-DNA
nicking. Furthermore, it was non-toxic at
concentrations ranging from 5 - 100 µg/ml.
Conclusion:
In vitro, phytooxyresveratrol (POV) extracted from
Artocarpus lakoocha exerted effective anti-aging
activity at a concentration of 25 µg/ml. Thus, the
substance should be further studied for possible
formulation into pharmaceutical products.
Keywords:
Aging, Artocarpus lakoocha, Free radicals, Glycation,
Phytooxyresveratrol, DNA nicking.
