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Research Article
Green Synthesis of Silver
Nanoparticles Induced by the Fungus Penicillium citrinum
Soheyla Honary1*,
Hamed Barabadi1, Eshrat Gharaei-Fathabad1
and Farzaneh Naghibi2
1Mazandaran University of
Medical Sciences, School of pharmacy, Sari, and 2School
of Traditional Medicine, Shaheed Beheshti University of
Medical Sciences, Tehran, Iran.
*For correspondence: Email:
shonary@mazums.ac.ir
or
shonary@yahoo.com
Tel: 0098 912 145 2220
Received: 5 April 2012
Revised accepted: 4 December 2012
Tropical
Journal of Pharmaceutical Research, February 2013;
12(1): 7-11
http://dx.doi.org/10.4314/tjpr.v12i1.2
Abstract
Purpose:
To evaluate a green process for the
extracellular production of silver (Ag) nanoparticles
synthesized and stabilized using Penicillium citrinum
isolated from soil.
Methods: The pure colonies of Penicillium citrinum
were cultured in Czapek dox broth. The supernatant of
the broth was examined for the ability to produce silver
nanoparticles. The reactions were performed in a dark
compartment at 28 ºC. After 24 h, the synthesized silver
nanoparticles were filtered through a membrane filter
(0.45 μ) and characterized by UV-visible spectroscopy,
fluorescence spectroscopy, photon correlation
spectroscopy (PCS), scanning electron microscopy (SEM)
and Fourier transformed infrared spectroscopy (FTIR) for
particle size, shape and the presence of different
functional groups in the nanoparticles.
Results: The silver nanoparticles formed were fairly
uniform in size with a spherical shape and a Z-average
diameter of 109 nm. FTIR spectra revealed the presence
of amide linkage groups which were also found in the
fungal extract itself.
Conclusion: The current approach suggests that rapid
synthesis of nanoparticles of silver nitrate would be
suitable for developing a biological process for mass
scale production of formulations.
Keywords: Green synthesis,
Penicillium citrinum, silver nanoparticles. |
