Mechanism of Ursolic
Acid-Mediated Inhibition of Proliferation in Vascular
Endothelial Glioaytoma
Lin-Qi Ye1,
Xiu-Feng Ye2 and Hong Xu3*
1Life Science and Technology
Institute of Yangtze Normal University,
Chongqing,2Department
of Pathology, Chongqing Medical University, Chongqing,
China,3Karolinska Institutet, Onco Reg AB,
Sweden.
*For correspondence:
Email:
xuhong10@126.com
Phone/Fax: +86-23-72374466
Received: 12 June 2012
Revised accepted: 28 July 2013
Tropical Journal of
Pharmaceutical Research, August 2013; 12(4): 511-515
http://dx.doi.org/10.4314/tjpr.v12i4.10
Abstract
Purpose: To investigate the effects
of ursolic acid (UA) on expressions of ERK1, C-Jun, C-Myc
and Cyclin D1 in Human Umbilical Vein Endothelial Cells
(HUVEC), and to explore the mechanism of anti-cancer
activity of UA on glioma.
Methods:
HUVEC was treated with UA
(0, 31.5, 62.5, 125, 250, 500 μg/mL) for 24 h, and 125
μg/mL for 0, 12, 24, 48 h, respectively) and PD98059 in
vitro. Real-time polymerase chain reaction (RT-PCR) was
performed to measure the endogenous mRNA levels of ERK1,
C-Jun, C-Myc, and Cyclin D1, and Western blotting was
used to determine the expressions of ERK1, C-Jun, C-Myc,
and Cyclin D1 proteins.
Results:
The results show that the
mRNA levels of ERK1, C-Jun, C-Myc, and Cyclin D1 were
down-regulated, following treatment with UA (in a dose-
and time-dependent manner) and PD98059 (p < 0.05). In
addition, the protein expressions of ERK1, C-Jun, C-Myc,
and cyclin D1 were all significantly down-regulated,
after treatment with UA (in a dose- and time-dependent
manner) and PD98059 (p < 0.05).
Conclusion:
The findings indicate that
UA can significantly inhibit the generation of vascular
endothelial cells of glioma by down-regulating the
expressions of ERK1, C-Jun, C-Myc and Cyclin D1 of ERK
signal transduction pathway.
Keywords:
Ursolic acid, Vascular
endothelial cell, ERK signal pathway, Glioma,
Down-regulation, Anti-cancer.
