Inhibitory Effect of Polysaccharides from Scutellaria
barbata D. Don on Invasion and Metastasis of 95-D
Cells Lines via Regulation of C-MET and E-CAD
Expressions
Xiao-Kun Yang1,2, Ya-Dong
Yang1,
Shu-Qian Tang1, Ling
Xu1, Gui-Hong
Yang1, Qiao-Yu
Xu1, Hui
Tang1 and Jin-Jin Wu1*
1Department
of Dermatology, Research Institute of Wound Healing and
Tissue Engineering of Chongqing, Daping Hospital, The
Third Military Medical University, Chongqing, 400042,
2Department
of Emergency, General Hospital of Chengdu Military
Command Area, Chengdu 610083, Sichuan Province, PR China
*For correspondence:
wujjtmmu@126.com
Tel: +86
023-68757599 ; Fax :
+86
023-68757599
Received: 17 April 2013
Revised accepted: 3July
2013
Tropical Journal of
Pharmaceutical Research, August 2013; 12(4): 517-522
http://dx.doi.org/10.4314/tjpr.v12i4.11
Abstract
Purpose: To
investigate the inhibitory effect of polysaccharides
from Scutellaria barbata (PSB) on invasion and
metastasis of lung cancer, and study the possible
mechanism.
Methods:
PSB was
extracted with water and by alcohol
precipitation, and purified by DEAE-52 column
chromatography. A highly invasive and metastatic lung
carcinoma cell, 95-D cell line, was used for the study.
Cell adhesion and invasion assays in vitro were
performed to evaluate the anti-invasive and anti-metastatic
effects of PSB (50 - 200 μg/ml) on 95-D cell.
Immunocytochemical staining and Western blot techniques
were employed to study the regulatory effects of PSB on
the expression of C-MET and E-CAD.
Results:
The
results indicate that PSB significantly inhibited cell
invasion and migration of 95-D in a
concentration-dependent manner (p < 0.05). The adhesion
of 95-D cells to fibronectin was also inhibited by PSB
(p < 0.05). The expression of C-MET and E-CAD in 95-D
cells treated with PSB were significantly down-regulated
and up-regulated, respectivelt (p < 0.05).
Conclusion: Treatment
with PSB can significantly inhibit the invasion and
metastasis of 95-D cells in vitro, probably through the
regulation of C-MET and E-CAD.
Keywords:
Polysaccharide,
Scutellaria barbata, 95-D cell lines, Invasion,
Metastasis
