Tropical Journal of Pharmaceutical Research

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Original Research Article

Quercetin - A Flavonoid Compound from Sarcopyramis bodinieri var delicate with Potent ial Apoptotic Activity in HepG2 Liver Cancer Cells

Weifeng Huang², Chunpeng Wan¹* and Shouran Zhou³

¹Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ²Medical College, China Three Gorges University, Yichang 443002, ³Jiangxi University of Traditional Chinese Medicine, Nanchang 330006, China

*For correspondence: Email: lemonwan@126.com Tel: +86 791 83813185; Fax: +86 791 83813185;

Received: 25 April 2013 Revised accepted: 25 July 2013

Tropical Journal of Pharmaceutical Research, August 2013; 12(4): 529-533

http://dx.doi.org/10.4314/tjpr.v12i4.13

Abstract

Purpose: To isolate and identify the cytotoxicity of the constituents of Sarcopyramis bodinieri var. delicate.

Methods: S. bodinieri var. delicate was extracted with hydrochloric acid-methanol and fractionated with ethyl acetate further. The chemical constituents of the ethyl acetate fraction were purified by a combination of D101 macroporous resin and Sephadex LH-20 column chromatography. The structure was characterized by ¹H-Nuclear Magnetic Resonance (NMR) and electrospray ionization tandem mass spectrometry (ESI-MS). Apoptosis was evaluated by fluorescence staining and Western blot analysis using 4,6-diamidino-2-phenylindole (DAPI) staining and poly (ADP-ribose) polymerase (PARP) SDS-PAGE tests in HepG2 liver cancer cells.

Results: One flavonoid with high purity was purified by the combination of D101 macroporous resin and Sephadex LH-20 column chromatography. The flavonoid compound was identified as quercetin by ¹H-NMR and ESI-MS analyses. DAPI staining and PARP SDS-PAGE tests showed 60 mM quercetin could induce potential apoptotic activity in HepG2 liver cancer cells.

Conclusion: Quercetin was the major cytotoxicity constituent in S. bodinieri var. delicate.

Keywords: Apoptotic activity, Quercetin, Sarcopyramis bodinieri var. delicate, HepG2 liver cancer cells, Electrospray ionization tandem