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Original Research Article
Larvicidal activity of
Illicium difengpi BN Chang (Schisandraceae) Stem
Bark and its Constituent Compounds against Aedes
aegypti L
Ying Liu1,2,3,
Xin Chao Liu1, Qi Yong Liu2, Chang
Niu3 and Zhi Long Liu1*
1Department of Entomology,
China Agricultural University, 2 Yuanmingyuan West Road,
Haidian District, Beijing 100193, 2State Key
Laboratory for Infectious Disease Prevention and
Control, National Institute for Communicable Disease
Control and Prevention, Chinese Center for Disease
Control and Prevention, Beijing, 102206, 3College
of Life Sciences, Capital Normal University, Haidian
District, Beijing 100048, China
*For correspondence:
Email:
zhilongliu@cau.edu.cn; Tel:
+86-10-62732800; Fax: +86-10-62732800
Received: 2 August 2014
Revised accepted: 15
December 2014
Tropical Journal of Pharmaceutical Research,
January 2015;
14(1): 103-109
http://dx.doi.org/10.4314/tjpr.v14i1.15
Abstract
Purpose: To determine the larvicidal
activity of the essential oil derived from Illicium
difengpi B.N. Chang stem bark (Schisandraceae) and its
major constituents against the larvae of Aedes aegypti
L.
Methods: Essential oil of I.
difengpi stem bark was obtained by hydrodistillation and
analyzed by gas chromatography (GC) and gas
chromaotography-mas spectrometry (GC-MS). The activity
of the essential oil and its major constituents was
evaluated, using World Health Organization (WHO)
procedures, against the fourth instar larvae of A.
aegypti for 24 h, and larval mortalities recorded at
essential oil/compound concentrations ranging from 6.0 -
200 μg/mL.
Results: A total of 36 components of
the essential oil of I. difengpi were identified. The
principal compounds are safrole (18.21 %), linalool
(13.47 %), 1,8-cineole (12.84 %), and myristicin (8.06
%) followed by α-terpineol (4.77 %), β-pinene (4.45 %)
and 4-terpineol (4.38 %). The essential oil exhibited
larvicidal activity against A. aegypti with LC50
(median lethal concentration) of 31.68 μg/mL. The major
constituents, myristicin, safrole, and 1, 8-cineole,
exhibited LC50 of 15.26, 39.45, and 72.18 μg/mL,
respectively.
Conclusion: The findings obtained
indicate that the essential oil of I. difengpi and its
major constituents have potentials for use in the
control of A. aegypti larvae and may therefore be useful
in the search for newer, safer and more effective
natural compounds as larvicides.
Keywords: Illicium difengpi, Aedes
aegypti, Larvicidal activity, Myristicin, Safrole,
1,8-Cineole, Linalool |
