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Original Research Article
Determination of
Antioxidant and Anti-Melanogenesis Activities of
Indonesian Lai, Durio kutejensis [Bombacaceae (Hassk)
Becc] Fruit Extract
Enos Tangke Arung1*,
Wiwin Suwinarti1, Medi Hendra2,
Supomo3, Irawan Wijaya Kusuma1,
Dwi Caesarina Nanda Puteri1, Huseyin Avni
Eroglu4, Yong-ung Kim5, Kuniyoshi
Shimizu6 and Hiroya Ishikawa7*
1Faculty of Forestry, 2Faculty
of Mathematics and Science, Mulawarman University,
3Samarinda Academy of Pharmacy, Samarinda, 75123,
Indonesia, 4Faculty of Veterinary Medicine,
Department of Physiology, University of Kafkas, Turkey,
5Department of Herbal Pharmaceutical
Engineering, College of Herbal Bio-industry,Daegu Haany
University, Gyeongsangbuk-do, South Korea, 6Faculty
of Agriculture, Kyushu University, 7International
College of Arts and Sciences, Fukuoka Women's
University, Fukuoka, 813-8529, Japan
*For correspondence:
Email:
tangkearung@yahoo.com,
ishikawa@fwu.ac.jp; Tel/Fax:
+62-541-737081, +81-92-661-2411/2415
Received: 14 May 2014
Revised accepted: 27
November 2014
Tropical Journal of Pharmaceutical Research,
January 2015;
14(1):
41-46
http://dx.doi.org/10.4314/tjpr.v14i1.7
Abstract
Purpose: To investigate the
antioxidant and anti-melanogenesis activities of Durio
kutejensis [Bombacaceae (Hassk.) Becc] fruit extract.
Methods: The fruit flesh of D.
kutejensis was extracted successively with n-hexane,
ethyl acetate/EtOAc, and ethanol/EtOH at room
temperature repeatedly. The extracts were concentrated
in vacuo to yield their residues. Antioxidant properties
were analyzed by
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)
(ABTS, superoxide dismutase (SOD)-like activity,
2,2-diphenyl-1-picrylhydrazyl (DPPH), and oxygen radical
absorbance capacity (ORAC) while anti-melanogensis
activity was evaluated by tyrosinase enzyme activity and
B16 melanoma cell assays (melanin inhibition and
cytotoxicity).
Results: The extract (200 µg/mL)
showed melanin inhibition by inhibiting melanin
formation in B16 melanoma cell by 47 % without
cytotoxicity but did not inhibit tyrosinase enzyme
activity. The extract (1 - 1000 µg/mL) also exhibited
some level of antioxidant activity including ORAC (0.04
± 0.00 µmol TE/mg at 950 µg/mL), ABTS (1.0 ± 0.2 % at
100.8 µg/mL), SOD (IC50, 76.00 ± 14.6 µg/mL, and DPPH
(21.5 ± 0.7 % at 97.39 µg/mL extract concentration).
Conclusion: The fruit extract of Durio
kutejensis has antioxidant properties with a potential
for treating hyperpigmentation and for use as a
skin-lightening agent.
Keywords: Durio kutejensis,
Antioxidant, Anti-melanogenesis, B16 Melanoma cell
Hyperpigmentation, Skin-lightening agent |
