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Original
Research Article
A New Flavanone from Flemingia strobilifera
(Linn) R. Br. and its Antimicrobial Activity
S Madan*a, GN Singha, Y Kumarb,
K Kohlic, R M Singha, SR Mirc
and S Ahmadc
aCentral
Indian Pharmacopoeia Laboratory, Govt. of India,
Ministry of Health and Family welfare, Sector – 23, Raj
Nagar, Gaziabad, U.P. – 201002, India
bI.T.S.
Paramedical College (Pharmacy), Delhi-Meerut Road, Murad
Nagar, Ghaziabad, U.P. – 2012006, India
cDepartment
of Pharmacognosy and Phytochemistry, Faculty of
Pharmacy, Hamdard University, New Delhi – 110062, India.
Tropical
Journal of Pharmaceutical Research, March
2008; 7(1):
921-927
Abstract
Purpose:
To carry out a bioactivity guided fractionation and
isolation of the antimicrobial constituent(s) of the
roots of Flemingia strobilifera against some bacteria
and fungi.
Methods:
The root of F. strobilifera was extracted with methanol,
butanol and dichloromethane. Antimicrobial activity of
the extracts was determined against both bacteria and
fungi while the isolation and characterization of
compounds of the extracts was done by column
chromatography,2D spectroscopic studies and spectral
data (U.V, I.R, NMR and MS).
Results:
Flemingiaflavanone
(8,
3'-diprenyl-5, 7, 4'-trihydroxy
flavanone), Genistin (5, 4'-dihydroxy
isoflavone 7-O-glucoside)
and β - sitosterol-D glucoside
were isolated from the extracts. Flemingiaflavanone
showed significant antimicrobial activity against
Gram-positive (S. aureus, S. epidermidis, MRSA),
Gram-negative bacteria (Ps. aeruginosa, E. coli) and
fungi (C. albicans). Genistin showed moderate activity
against Gram-positive, Gram-negative bacteria and fungi.
Conclusions:
The isolation and
antimicrobial activity of presence of Flemingiaflavanone
is being reported for the first time.
Key words: Flemingia
strobilifera, Flemingiaflavanone (8, 3'-diprenyl-5,
7, 4'-trihydroxy flavanone), Genistin (5, 4'-dihydroxy
isoflavone 7-O-glucoside), MeOH (Methanol), BuOH (Butanol),
Antimicrobial activity, MIC (Minimum inhibitory
concentration). |
