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Original
Research Article
Microstructural
Observations on Nissl Substances in the Cerebellar
Cortex of Adult Wistar Rats following Quinine
Administration
Adesina J Ajibade*1, Olusola A Adeeyo1,
David A Ofusori2, Thomas K Adenowo3,
Olufunto O Ishola4, Ebenezer A Ashamu1
and Spencer C Nwangwu 5
1Department
of Anatomy, Faculty of Basic Medical
Sciences, Ladoke Akintola University of
Technology, Ogbomoso; 2Department
of Anatomy and Cell Biology, Faculty of
Basic Medical Sciences, Obafemi Awolowo
University, Ile-Ife;
3Department
of Anatomy, Faculty of Basic Medical
Sciences, Olabisi Onabanjo University, Ago-Iwoye;
4Department of Physiology,
Faculty of Basic Medical Sciences, Ladoke
Akintola University of Technology, Ogbomoso;
and 5Department of Bi ochemistry,
School of Basic Medical Sciences, Igbinedion
University, Okada, Nigeria.
Received:
22
September 2008
Revised accepted:
5 December 2008
Tropical
Journal of Pharmaceutical Research, April
2009; 8(2):
105-109
Abstract
Purpose:
This study assessed some microstructural effects of
quinine, commonly used in malaria chemotherapy,
especially in chloroquine-resistant and cerebral
malaria, on the Nissl substance in the cerebellar cortex
of adult Wistar rats using microanatomical studies.
Methods:
Twenty seven adult male Wistar rats, weighing between
150g and 190g, were randomly separated into groups A, B
and C (n=9). The rats in group A served as the control
and received intramuscular injection of physiological
saline. Group B rats were injected intramuscularly with
liquid quinine, 16mg/kg body weight as a start dose,
followed by 8mg/kg body weight 8 hourly for seven days.
Group C rats received the same treatment as group B but
were subjected to a withdrawal period of one week.
Groups A and B rats were sacrificed at the end of the
treatment while group C rats were sacrificed at the end
of one week. The cerebellum of each rat was removed and
fixed in 10% formol saline for histological analysis.
Results:
The findings showed that the Nissl substances in the
cerebellar cortex in control rats stained more intensely
and distinctly compared with the less intense stain and
degenerated Nissl substances in the treated rats.
Conclusion:
The observed degenerative changes in the Nissl
substances in the cerebellar cortex of the treated rats
may affect the synthesis of proteins in correlation with
neuronal functions.
Keywords: Quinine, Nissl substances, Intensely
stained, Cerebellar cortex, Degenerative changes |
