|
|
Original
Research Article
In
vitro
Anti-Leishmanial and Anti-Tumour Activities
of a Pentacyclic Triterpenoid Compound
Isolated from the Fruits of Dregea
volubilis Benth Asclepiadaceae
Biswas Moulisha1*, Mandal
Nirup Bikash2, Palit Partha2,
Ghosh Ashoke Kumar3, Bannerjee
Sukdeb2 and Haldar Pallab.Kanti4
1Bengal
Institute of Pharmaceutical Sciences,
Kalyani, Nadia-741 235, 2Indian
Institute of Chemical Biology, Department of
Steroids and Terpenoids Chemistry,
Jadavpur-700 032, ,3Bengal School
of Technology, Delhi Road, Sugandha, Dt.
Hooghly, PIN: 712 102, 4Division
of Pharmacology, Department of
Pharmaceutical Technology, Jadavpur
University, Kolkata - 700 032, West Bengal,
India.
E-mail:
moulisha_biswas@yahoo.co.in Tel:
+91-33-25211273; +91-9831354803(M); Fax:
+91-33-25891742
Received:
16
September 2008
Revised accepted:
5
January 2008
Tropical
Journal of Pharmaceutical Research, April
2009; 8(2):
127-131
Abstract
Purpose:
Dregea volubilis
Benth, commonly
known as Jukti in Bengal, is used in the treatment of
boils and abscesses from ancient times. The purpose of
this study is to elucidate the active compounds and as
well as their anti-leishmanial and anti-tumour
activities.
Methods:
Dried and crushed fruits of Dregea volubilis were
extracted by petroleum ether (40 - 60°C); the best
solvent system had first been verified by analytical
Thin Layer Chromatography (TLC). The extract was
subjected to TLC and column chromatography (CC) to
isolate the pure compounds. Spectra data were obtained
by Infra Red
pectroscopy, Mass Spectroscopy and Nuclear Magnetic
Resonance - Proton Magnetic Resonance (PMR), Carbon
Magnetic Resonance (CMR) and Distortionless Enhancement
by Polarization Transfer (DEPT) - for structure
elucidation of the isolated compound(s). One of the
compounds isolated was screened for anti-leishmanial
activity against promastigotes of Leishmania donovani
and anti-tumour activity on K562 leukemic cell line.
Results:
A pentacyclic triterpenoid compound was isolated and
designated as taraxerone, and then characterized as
d-friedoolean-14-en, 3 one together with ß-sitosterol
and a long chain lipid fraction.. This compound showed
in vitro anti-leishmanial activity against promastigotes
of Leishmania donovani (strain AG 83) and anti-tumour
activity on K562 leukemic cell line.
Conclusion:
A pentacyclic triterpenoid compound designated as
taraxerone and characterized as D-friedoolean-14-en, 3
one together was successfully isolated. The structure
was determined on the basis of spectral analysis (IR,
MASS, NMR (PMR, CMR and DEPT) and the compound
demonstrated in vitro anti-leishmanial and anti-tumour
activities.
Keywords:
Dregea volubilis, Pentacyclic
triterpenoid, Taraxerone, Spectroscopy (IR, MASS, CMR,
PMR, DEPT), Anti-leishmanial, Anti-tumour. |
