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Research
Article
Antioxidant Activity of the Bulb and Aerial Parts of
Ornithogalum sintenisii
L (Liliaceae)
at Flowering Stage
Mohammad Ali Ebrahimzadeh1*, Seyed Mohammad
Nabavi1,2, Seyed Fazel Nabavi1 and
Bahman Eslami3
1Pharmaceutical Sciences Research Center, School of Pharmacy, Mazandaran
University of Medical Sciences, 48189, Sari, 2Department
of Biology, University of Mazandaran, Babolsar,
3Department of Biology, Islamic Azad University, Ghaemshahr Branch,
Iran.
*Corresponding author:
E-mail: zadeh20@yahoo.com; Tel:
+98-151-3543081-3; Fax: +98-151-354308
Received: 17 August 2009
Revised
accepted: 17 February 2010
Tropical
Journal of Pharmaceutical Research, April 2010;
9(2):
141-148
Abstract
Purpose:
Ornithogalum sintenisii
is an Iranian species with little known about its
pharmacological effects. The purpose of the present
study was to investigate some antioxidant properties of
the plant.
Methods: The antioxidant potency of the freeze-dried methanol
extract of
O. sintenisii
bulbs and aerial parts were investigated by evaluating
the following parameters: linoleic acid peroxidation,
1,1-diphenyl-2-picryl hydrazyl radical (DPPH),
scavenging of nitric oxide and hydrogen peroxide as well
as reducing power and Fe2+ chelating
activity, using standard methods. Phenol and flavonoid
contents were determined as gallic acid and quercetin
equivalents, respectively.
Results: The aerial parts
contained higher phenol and flavonoid contents than the
bulbs. IC50 for DPPH
radical-scavenging activity was 368 ± 15 and 669 ± 25 µg
ml-1 for aerial parts and bulbs,
respectively.
The reducing power of the extracts was less than that of
vitamin C (p < 0.01) with the aerial parts showing
stronger activity than the bulbs (p < 0.01). The
extracts did not show any activity in the peroxidation
test but displayed good H2O2
radical scavenging activity compared with quercetin (IC50=
52.0± 3.1
mg ml-1) which was used as positive control.
Conclusion:
The bulb and aerial parts of
O. sintenisii aerial parts
(at flowering stage)
exhibited good but varying levels of antioxidant
activities in nearly all the models studied.
Keywords:
Ornithogalum sintenisii ; Bulbs; Aerial parts; Antioxidant activity;
Flavoniods; Phenols. |
