Inhibition
of Pro-inflammatory Cytokines by Ethyl Acetate Extract
of Scrophularia striata
Abbas
Azadmehr1, Ghorban Maliji2*, Reza
Hajiaghaee3, Mojtaba Shahnazi4 and
Ahmad Afaghi5
1Immunology Department, Qazvin University of Medical
Sciences, Qazvin, 2Immunology Department,
Babol University of Medical Sciences, Babol,
3Department of
Pharmacognosy & Pharmaceutics Department of Medicinal
Plants Research Center, Institute of Medicinal Plants,
ACECR, Karaj, Iran, 4Parasitology Department, 5Social
Medicine Department, Qazvin University of Medical
Sciences, Qazvin, Iran.
*For correspondence:
Email:
aazadmehr@qums.ac.ir
ghmaliji@yahoo.com
Tel: +98-1112229591-5; Fax: 98-1112229591
Received:
7
November 2011
Revised
accepted: 15 October 2012
Tropical
Journal of Pharmaceutical Research, December 2012;
11(6): 893-897
http://dx.doi.org/10.4314/tjpr.v11i6.4
Abstract
Purpose: To investigate the effect of ethyl
acetate extract of S. striata on pro-inflammatory
production by macrophages.
Methods: Mouse peritoneal macrophages were
cultured in solvent either alone or with 2 µg/ml
lipopolysaccaride (LPS) with/without different doses of
ethyl acetate extract of S. striata. Production of
pro-inflammatory cytokines including interleukin 1β
(IL-1β), tumor necrosis factor - α (TNF-α) and
prostaglandin E2 (PGE2) were examined using ELISA.
Results: Ethyl acetate fraction of S. striata
in doses of 10, 50, 100 and 200 µg/ml significantly (p <
0.05) inhibited pro-inflammatory mediators (IL-1β, TNF-α,
and PGE2) production by LPS stimulated peritoneal
macrophages.
Conclusion: The anti-inflammatory effect of the extract on
pro-inflammatory cytokines may ameliorate inflammatory
diseases, possibly via an immunomodulatory mechanism.
Keywords: Pro-inflammatory, Cytokines, Ethyl
acetate extract, Scrophularia striata
