Development and Validation
of Analytical Method for Losartan-
Copper Complex Using UV-Vis
Spectrophotometry
Syed S ul Hassan1,
Syeda FA Zaidi1, Imran Tariq1* and Muhammad T Ansari2
1University College of
Pharmacy, University of the Punjab, Lahore-54000, 2Department
of Pharmacy, Bahaudin Zakariya University, Multan,
Pakistan
*For correspondence:
Email:
imran_1982@hotmail.com Tel:
0092-322-4218583, 0092-42-36156006
Received: 3 August 2012
Revised accepted:
26 April 2013
Tropical Journal of
Pharmaceutical Research, June 2013;
12(3): 407-411
http://dx.doi.org/10.4314/tjpr.v12i3.20
Abstract
Purpose: To develop a new
spectrophotometric method for the analysis of losartan
potassium in pharmaceutical formulations by making its
complex with copper.
Method: A coloured complex based
on UV/Vis spectroscopic method was developed for the
determination of losartan potassium concentration in
pharmaceutical formulations in the visible region. The
colored complex of losartan was formed with cupric
acetate (5:4). Analysis was carried out by the two
methods - absorption ratio and calibration curve
methods. The proposed method was validated in terms of
linearity, accuracy and precision.
Results: The λ maximum of the
complex was recorded at 530 nm. Beer`s law was obeyed
in the range of 10 - 50 µg/ml with a correlation
coefficient (r2) of 0.9989. Mean analytical
results from the calibration curve and absorption ratio
methods were 99.42 and 99.16 % with relative standard
deviation (RSD) of 0.97 and 0.82 %, respectively. Mean
recovery was between 98.61 and 101.35 % and precision
expressed as relative standard deviation (RSD) was 0.91
%.
Conclusion: The proposed method is
simple, easy to apply, low-cost, and requires relatively
inexpensive instruments. Thus, it is a suitable
alternative to currently used spectrophotometric methods
for the determination of losartan in bulk and solid
dosage forms.
Keywords:
Losartan, Copper complex,
Spectrophotometry, Validation
