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Original Research Article
Simultaneous Determination
of Co-formulated Matrine and Secnidazole
in Suppositories by Reverse
Phase High Performance Liquid Chromatography
Haixia
Lv, Suying Ma*, Xiao
Wang and Xiaojun Shang
School of
Pharmacy, Xinxiang Medical University, Xinxiang Henan,
453003, PR China
*For correspondence:
Email:
suyingma@163.com
Tel: + 86-373-3029879
Received: 20 February 2012
Revised accepted: 28 March 2013
Tropical Journal of
Pharmaceutical Research, June 2013;
12(3): 413-418
http://dx.doi.org/10.4314/tjpr.v12i3.21
Abstract
Purpose:
To develop and validate a
new, simple, sensitive and accurate high performance
liquid chromatographic (HPLC) method for the
simulataneous determination of matrine and secnidazole
in suppositories.
Methods:
The method involved using
a SinoChoom ODS-BP C18 column (5 μm, 4.6 mm ×
200 mm) and mobile phase consisting of acetonitrile–triethylamine
(0.05 %) in 0.025 mol/L KH2PO4
(20:80, v/v). The flow rate was 1 mL/min and detection
was monitored at 210 and 311 nm for matrine and
secnidazole, respectively. Total run time was 10 min and
the column was maintained at 35 oC.
Results:
The excipients in the
suppository did not interfere with the drug peaks.
Matrine was eluted at a retention time (RT) of 4.30 min
while linearity for the quantification of drug was
obtained in the concentration range of 10.0 - 100.0 µg/mL
(r2 = 0.9991). Secnidazole was eluted at a
retention time (tr) of 6.69 min and linearity for the
quantification of the drug was obtained in the
concentration range of 10.0 - 150.0 µg/mL (r2
= 0.9993). Intra- and inter-day variations were < 1.0 %
for both matrine and secnidazole.
Conclusion:
The developed HPLC method
was validated according to International Conference on
Harmonisation (ICH) guidelines and proved to be suitable
for the simultaneous determination of matrine and
secnidazole in suppositories.
Keywords:
Matrine, Secnidazole,
Suppository, HPLC, Assay |
