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Original Research Article
Ultrastructural Study of
Elaeis guineensis (Oil Palm) Leaf and
Antimicrobial Activity of its Methanol Extract against
Staphylococcus Aureus
Amala Rajoo1,
Sreenivasan Sasidharan2*, Subramanion L Jothy2,
Surash Ramanathan1 and Sharif M Mansor1
1Centre for Drug Research,
2Institute for Research in Molecular Medicine
(INFORMM), Universiti Sains Malaysia, 11800 Minden,
Penang, Malaysia.
*For correspondence:
Email:
srisasidharan@yahoo.com
Received: 18 July 2012
Revised
accepted: 12 April 2013
Tropical Journal of
Pharmaceutical Research, June 2013;
12(3): 419-423
http://dx.doi.org/10.4314/tjpr.v12i3.22
Abstract
Purpose:
To evaluate the
antimicrobial activity of the methanol extract of
Elaeis guineensis leaf against Staphylococcus aureus and
to determine the effect of extract treatment on the
microstructure of the microbe
Methods: The antimicrobial
activity of the methanol leaf extract of the plant
against S. aureus was examined using disc diffusion and
broth dilution methods. Scanning electron microscopy (SEM)
and transmission electron microscopy (TEM) were carried
out to determine the major alterations in the
microstructure of S. aureus after treatment with the
extract.
Results: The extract showed a
good antimicrobial activity against S. aureus with a
minimum inhibition concentration (MIC) of 6.25 mg/mL and
for Chloramphenicol was 30.00 µg/mL. The main changes
observed under SEM and TEM were structural
disorganization of the cell membrane which occurred
after 12 h and total collapse of the cell 36 h after
exposure to the extract.
Conclusion:
We concluded that the
methanolic extract of E. guineensis leaf exhibited good
antimicrobial activity against S. aureus and this is
supported by SEM and TEM.
Keywords:
Antimicrobial activity,
Elaeis guineensis, Staphylococcus aureus, Scanning
electron microscopy, Transmission electron microscopy. |
