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Original Research Article
Antioxidant Activity of
Pandanus amaryllifolius Leaf and Root Extract and
its Application in Topical Emulsion
Ampa Jimtaisong* and Panvipa
Krisdaphong
School of
Cosmetic Science, Mae Fah Luang University, 333 Moo1,
Thasud, Muang, Chiang Rai, 57100 Thailand
*For correspondence:
Email:
ampa@mfu.ac.th
Tel: +6653916843; Fax: + 6653916831.
Received: 18 June 2012
Revised accepted:
4 April 2013
Tropical Journal of
Pharmaceutical Research, June 2013; 12(3): 425-431
http://dx.doi.org/10.4314/tjpr.v12i3.23
Abstract
Purpose: To develop Thai pandan (Pandanus
amaryllifolius) as an antioxidant ingredient for topical
emulsion.
Methods: Dried leaf and root of
P. amaryllifolius (Pandanceae) were extracted separately
by maceration with either ethanol or propylene glycol.
Antioxidant capacity was investigated by
2,2-diphenyl-1-picry hydrazyl radical (DPPH) and
linoleic acid peroxidation method. The total phenolic
content was measured by Folin-Ciocalteau assay.
Oil-in-water topical emulsions containing the individual
extracts were prepared and tested for stability.
Results: Propylene glycol extract
exhibited higher DPPH activity and total phenolic
content than the ethanol extract while the DPPH activity
of the leaf extract was higher than that of the root.
The 50 % inhibition concentration (IC50)
value of leaf and root extracts was 0.810 and 2.340
mg/ml, respectively. Although the antioxidant activity
of the crude extracts was lower than that of standard
vitamin C and butylated hydroxytoluene
(BHT), the ethanol/propylene glycol solution
extract (ethanol was reduced to 50 % extract volume)
showed higher inhibition (90.1 %) of linoleic acid
peroxidation than 200 ppm of vitamin C (57.1 %) and BHT
(71.1 %). An oil-in-water emulsion containing 3 % of
the ethanol and propylene glycol extract showed creamy
texture with medium viscosity and demonstrated good
stability under accelerated aging test.
Conclusion: The results indicate a
potential for the development of P. amaryllifolius leaf
extract as an antioxidant ingredient in topically
applied formulations.
Keywords: Pandanus amaryllifolius,
Extraction, Antioxidant, Emulsion, Stability. |
