Preparation of Gold
Nanoparticles for Biomedical Applications Using
Chemometric Technique
Soheila
Honary1*, Pouneh Ebrahimi2 and
Maedeh Ghasemitabar1
1Mazandaran University of Medical Sciences, School
of Pharmacy, Pharmaceutical Sciences Research Center,
Sari, 2Gonbad-Kavous University, PO Box 163,
Gonbad, Iran
*For correspondence:
Email:
Shonary@yahoo.com
or
honary@ualberta.ca
Received: 6 July 2012
Revised accepted:
31 March 2013
Tropical Journal of
Pharmaceutical Research, June 2013;
12(3): 295-298
http://dx.doi.org/10.4314/tjpr.v12i3.3
Abstract
Purpose: To study the effect of
process conditions on the size of gold nanoparticles (AuNPs)
prepared by chemometric technique.
Methods: AuNPs were prepared by
adding 5 ml of 5 mM of gold (III) chloride hydrate
HAuCl4 (2 mg/mL)
to 85 ml of filtered deionized water, then refluxed in a
250 mL flask over a hot plate and heated to boiling
point. Five milliliters of sodium citrate solution of
varying concentrations were quickly added to the boiling
solution and stirred for 30 min until the color turned
to wine red. Chemometric approach, based on multivariant
analysis, was applied to the optimization of iron oxide
nanoparticle size in respect of three parameters, viz,
concentration of sodium citrate solution, stirrer speed
and ionic strength of the medium. The experiments were
performed according to Box-Behnken experimental design.
Results: The regression model
obtained was characterized by both descriptive and
predictive ability. The method was optimized with
respect to average diameter as a response. The average
diameter of the nanoparticles produced under different
conditions were between 17.7 up to 168.8 nm. The
criteria for the evaluation of the descriptive
capability of a polynomial were R2 = 0.974,
standard error = 13.994 and F-ratio = 18.4.
Conclusion: It can be concluded that
the Box-Behnken experimental design provides a suitable
approach for optimizing and testing the robustness of
gold nanoparticle preparation method.
Keywords:
Box-Behnken design,
Optimization, Nanoparticles, Gold, Biomedical,
Chemometric.
