Isolation of Antidiabetic
Principle from Bougainvillea spectabilis Willd (Nyctaginaceae)
Stem Bark
Sunil Jawla1*, Yatendra Kumar1
and Mohammad Sardar Yar Khan2
1Department of Pharmaceutical
Chemistry, I.T.S. Paramedical College (Pharmacy), Delhi-Meerut
Road, Muradnagar, Ghaziabad, 201206.
2Department of Pharmaceutical
Chemistry, Faculty of Pharmacy, (Jamia Hamdard) Hamdard
University, New Delhi, India 110062.
*For correspondence:
Email:
suniljawla@its.edu.in; Tel:
+911232-225380; Fax: +911232-225380
Received:
31 January 2012
Revised accepted:
7 July 2013
Tropical Journal of
Pharmaceutical Research, October 2013;
12(5):
761-765
http://dx.doi.org/10.4314/tjpr.v12i5.15
Abstract
Purpose: To isolate and identify the
constituents of Bougainvillea spectabilis Willd (Nyctaginaceae)
stem bark.
Methods: The methanol extract of
Bougainvillea spectabilis stem bark powder was suspended
in water and extracted with dichloromethane (CH2Cl2),
ethyl acetate (EtOAc), and butanol (BuOH) successively.
The ethyl acetate fraction was loaded in a column packed
with silica gel and eluted with a gradient of chloroform
(CHCl3): methanol (MeOH), and water yielded
five fractions (A - E). Chemical constituents were
isolated by repeated column chromatography of these
fractions.
Results: Column chromatography of
fractions B and C afforded four compounds identified as
pinitol, β-sitosterol, quercetin and
quercetin-3-O-α-L-rhamnopyranoside. For the first time,
pinitol, β-sitosterol, quercetin and
quercetin-3-O-α-L-rhamnopyranoside were isolated from
the stem bark of B. spectabilis Willd.
Conclusion: An antidiabetic principle,
pinitol, was successfully isolated from the stem bark of
B. spectabilis Willd.
Keywords:
Bougainvillea spectabilis, Column chromatography,
Pinitol, Quercetin, Quercetin-3-O-α-L-rhamnopyranoside.
