Antioxidant and
Antimicrobial Activity of Polyphenol Extracts from Wild
Berry Fruits Grown in Southeast Serbia
Blaga C
Radovanović1, Ana S Milenković
Anđelković1*, Aleksandra B Radovanović1
and Marko Z Anđelković2
1Department of
Chemistry, Faculty for Mathematics and Science,
University of Niš, Višegradska
33, 18000 Niš,
2
CBRN Training Center,
Balkanska 57, 37000 Kruševac, Serbia
*For correspondence:
Email:
ana.andjelk@gmail.com; Tel:
+381 16 218415; Fax: +381 18 533014
Received: 17 October 2012
Revised accepted: 8 July 2013
Tropical Journal of
Pharmaceutical Research, October 2013;
12(5):
813-819
http://dx.doi.org/10.4314/tjpr.v12i5.23
Abstract
Purpose:
To assess the antioxidant and antimicrobial activities
of polyphenolic extracts of three wild red wild berry
fruit species from Southeast Serbia, viz, European
cornel (Cornus mas), blackthorn (Prunus
spinosa L.) and wild
blackberry (Rubus fruticosus).
Methods: Polyphenol content was
determined using spectrophotometric and High performance
liquid chromatography (HPLC methods. Antioxidant activity was
estimated by 2,2`- diphenyl - 1 - picrylhydrazyl (DPPH)
test while reliminary
antimicrobial tests were carried out by disc diffusion
method in which antibacterial
activity was evaluated by measuring the zone of
inhibition against test bacterial strains.
Broth microdilution method
was used to determine the minimum inhibitory
concentration (MIC) and minimum bactericidal
concentration (MBC).
Results: The highest amount of
total phenols was found in European cornel (8625.89 mg
kg-1FW). Galic acid, caffeic acid, p-coumaric
acid, ferulic acid, (+)-catechin, procyanidin B2, (-)-epicatechin,
quercetin, rutin and quercetin-3-glucoside were the main
polyphenols in the fruit extracts. All extracts showed
high scavenging effect on DPPH radical with IC50
values ranging from 22.19 to 31.18 ml g-1, as
well as high antimicrobial activity on almost all the
tested bacterial strains.
Conclusion:
Extracts of wild berry
fruits may be suitable for the preparation of medicinal
and nutritional products.
Keywords: European cornel,
Blackthorn, Wild blackberry, Polyphenols, Antioxidant,
Antimicrobial
