Anti-inflammatory Effects of Magnolia sieboldii Extract in Lipopolysaccharide-Stimulated RAW264.7 Macrophages

Baatartsogt Oyungerel¹, Heekyong Lim¹, Chi-Ho Lee², Eun-Hee Choi³, Guan-Hao Li⁴ and Kang-Duk Choi^1*   

¹Genomic Informatics Center, Hankyong National University, Anseong, ²Department of Food Science and Biotechnology of Animal Resources, College of Animal Bioscience and Biotechnology, Konkuk University, Seoul, ³Department of Medical science, College of Medicine, Dankook University, Cheonan, Korea, ⁴Department of Food Science, Agriculture College of Yanbian University, Jilin Province, China.

*For correspondence: Email: kchoi04@hknu.ac.kr; Tel: +82-316705422; Fax: +82-316705339

Received: 4 September 2013                                       Revised accepted: 10 October 2013

Tropical Journal of Pharmaceutical Research, December 2013; 12(6): 913-918

Purpose: To investigate the effect of Magnolia sieboldii extract (MSE) on the production of pro-inflammatory cytokines by macrophage.

Methods: The whole plant of M. sieboldii was extracted with methanol at room temperature. The in vitro anti-inflammatory activity of MSE was investigated on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. LPS-induced nitric oxide(NO) production was determined by Griess method. Production of pro-inflammatory cytokines including Interleukin-1 beta (IL-1 β), Interleukin-6 (IL-6), tumor necrosis factor- alpha (TNF-α) and cyclooxygenase-2 (COX-2) was examined using reverse trancriptase - polymerase chain reaction (RT-PCR) and Western blot analysis.

Results: Under in vitro conditions, MSE in doses ranging from 25 - 100 μg/mL significantly inhibited lipopolysaccharide (0.5 μg/mL)-induced nitric oxide production (p < 0.001), and the production of pro-inflammatory mediators (p < 0.05).

Conclusions: The anti-inflammatory effect of MSE on pro-inflammatory cytokines seems to ameliorate inflammatory symptoms via immune regulation.