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Original Research Article
Anti-Hyperlipidemic Effect
of Flavone-Rich Belamcanda chinensis (L.) DC. (Iridaceae)
Leaf Extract in ICR Mice Fed High-Fat Diet
Hai-Wei Zhao1,
Fang Lv1,2, Wei-Wei Meng1,2, Hao
Dang1, Zhi-Long Sun1, Yan Chen1,2,
Rong-Ji Dai1,2, Yu-Lin Deng1,2 and
Chong-Ming Wu3*
1School of Life Science,
Beijing Institute of Technology, Beijing 100081, 2Beijing
BIT&GY Pharmaceutical R&D, Beijing 100081, 3Institute
of Medicinal Plant Development, Chinese Academy of
Medical Science & Peking Union Medical College, Beijing
100193, PR China
*For correspondence:
Email:
wucm1979@gmail.com; Tel: +86
10 68949331; Fax: +86 10 68467208
Received: 24 July 2013
Revised accepted: 24 August
2014
Tropical
Journal of Pharmaceutical Research, October 2014;
13(10): 1653-1658
http://dx.doi.org/10.4314/tjpr.v13i10.12
Abstract
Purpose: To assess the anti-hyperlidemic
effect of flavone-rich B. chinensis leaf extract (HTP)
in ICR mice fed a high-fat diet.
Methods: HTP (100, 200 and 400
mg/kg) were orally administered to ICR mice fed high-fat
diet for 7 weeks. Blood glucose, as well as serum and
hepatic levels of lipids were determined at the end of
the experiment. Phosphorylation of adenosine 5′-monophosphate
(AMP)-activated protein kinase (AMPK) and protein level
of peroxisome proliferator-activated receptor α (PPARα)
were analyzed by Western blot and enzyme-linked
immunosorbent assay (ELISA) kit, respectively.
Results: Treatment with HTP
significantly decreased peri-epididymal fat weight (p <
0.01 and p < 0.05 for 200 and 100 mg/kg, respectively),
lowered serum and hepatic lipid, and decreased glucose
area under curve (AUC) in oral glucose tolerance test (p
< 0.01 for 200 mg/kg). Western blot and ELISA analysis
showed that administration of HTP (200 mg/kg)
significantly increased AMPK (p < 0.05) phosphorylation
and PPARα expression in liver (p < 0.05).
Conclusion: HTP can alleviate
hyperlipidemia, at least in part, by up-regulation of
AMPK and PPARα.
Keywords: Belamcanda chinensis,
Flavone, Hyperlipidemia, Adenosine 5′-monophosphate-activated
protein kinase, Peroxisome proliferator activated
receptor-alpha, Glucose tolerance |
