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Original Research Article
Diuretic Action of Exogenous Hydrogen Sulfide in
Spontaneously Hypertensive Diabetic Rats
Fiaz ud Din Ahmad1*, Munavvar A Sattar1,
Hassaan A Rathore1, Safia Akhter1,
Oh Hui Jin1 and Edward J Johns2
1School of Pharmaceutical
Sciences, Universiti Sains Malaysia, Minden, 11800
Penang, Malaysia, 2Department of Physiology,
Western Gateway Building, University College Cork, Cork,
Ireland
*For correspondence:
Email:
fazi2353@yahoo.com; Tel:
+60147575494
Received: 31 January 2014
Revised accepted: 8
September 2014
Tropical
Journal of Pharmaceutical Research, November 2014;
13(11): 1 867-1876
http://dx.doi.org/10.4314/tjpr.v13i11.14
Abstract
Purpose: To examine the hypothesis
that in hypertensive diabetic rats hydrogen sulphide (H2S)
reduces blood pressure through diuretic action in
addition to its vasodilating effect.
Methods: Spontaneously hypertensive
rats (SHR) and Wistar-Kyoto (WKY) rats were used. SHR
were divided into three groups: SHR (II), SHR diabetic
(III), and SHR diabetic NaHS-Treated (IV) with a group
of WKY (I) rats serving as normotensive nondiabetic
control. Diabetes was produced in two SHR groups using
intraperitoneal streptozotocin (STZ). One diabetic group
received NaHS, a donor of H2S (56 µM/kg i.p.)
daily for five weeks. Blood pressure was measured in
conscious and anesthetized states in surgically prepared
animals. Plasma and urinary H2S levels and
electrolytes were measured weekly throughout the 35-day
period.
Results: SHR and diabetic SHR had
higher blood pressure and lower plasma and urinary H2S
levels compared to WKY controls (p < 0.05). Moreover,
the SHR diabetic group had higher plasma sodium, higher
absolute and fractional sodium excretions (p < 0.05) but
with similar blood pressure compared to SHR controls.
NaHS treatment reduced blood pressure and restored H2S
and plasma sodium (p < 0.05) levels. Moreover, SHR
diabetic-NaHS treated group had higher urine output and
absolute urinary sodium excretion compared to the
untreated SHR diabetic group (p < 0.05).
Conclusion: These results suggest a
possible diuretic effect of exogenous H2S in
spontaneously hypertensive diabetic rats.
Keywords: Spontaneously hypertensive
rats, Diabetes, Hydrogen sulphide, Diuretic, Sodium
excretion, Urine output |
