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Original Research Article
Isolation of
(-)-Patchouli Alcohol from Patchouli Oil by
Fractional Distillation and
Crystallization
Zu Qing Su1,
Xiao Li Wu1, Mei Jia Bao2, Chu Wen
Li1, Song Zhi Kong1, Zi Ren Su1,3,
Xiao Ping Lai1,3, Yu Cui Li1* and
Jian Nan Chen1,3*
1School of Chinese Materia
Medica, Guangzhou University of Chinese Medicine,
Guangzhou 510006, 2Guangzhou Pharmaceutical
Vocational School, Guangzhou 511455, 3Dongguan
Mathematical Engineering Academy of Chinese Medicine,
Guangzhou University of Chinese Medicine, Songshan Hu
Industrial Park, Dongguan 523808, Guangdong, PR China
*For correspondence:
Email:
chenjiannan@gzucm.edu.cn,
liyucui85@163.com;
Tel: +86-20-39358517;
Fax: +86-20-39358390
Received: 13 August 2012
Revised accepted: 12
January 2014
Tropical Journal of
Pharmaceutical Research, March 2014;
13(3):
359-363
http://dx.doi.org/10.4314/tjpr.v13i3.7
Abstract
Purpose: To establish a new and
efficient method for the isolation of
(-)-patchouli alcohol (PA) from
patchouli oil (PO) .
Methods:
PO, obtained from commercial
source, was separated into four fractions (A, B, C and
raffinate) using fractional distillation according to
pre-set reflux ratio in vacuum. PA was crystallized from
fraction C (containing more than 80 % PA by weight) by
cooling and centrifugation. Finally, PA was further
purified by suction filtration. Characterization of PA
was performed by melting point (MP), infrared
spectroscopy (IR), 1H and 13C
nuclear magnetic resonance spectroscopy (NMR) and mass
spectrometry (MS).
Results:
The total yield of PA in
this procedure reached 52.9 %. The structure of PA was
obtained based on data from 1H-NMR, 13C-NMR
and MS analysis with the aid of literature data for
authenticated samples.
Conclusion:
Fractional distillation
combined with crystallization can be successfully
applied to the isolation of PA from PO in solvent-free
conditions.
Keywords:
Isolation, Patchouli
alcohol, Patchouli oil, Fractional distillation,
Crystallization
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