Tropical Journal of Pharmaceutical Research

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Original Research Article

Molecular Characterization of Glucose-6-Phosphate Dehydrogenase Deficiency in Abu Dhabi District, United Arab Emirates

Saleh Ahmed Bin Amro^1,2*, Eiman Al Zaabi²,Shakir Hussain², Amira Mahmoud Aly², Huda Salman Baqir¹,Abdul–Hamid Ahmed Zaki¹, Sultan Ayesh Mohammed Saghir³ andNarazah Mohammed Yusoff¹

¹Cluster of Regenerative Medicine, Advanced Medical and Dental Institute (AMDI), Universiti Sains Malaysia (USM), 11800 Penang, Malaysia, ²Hematopathology Section, Department of Pathology and Laboratory Medicine, Mafraq Hospital, Abu Dhabi, United Arab Emirates (UAE), ³School of Pharmacy, Pharmacology Department, University Sains Malaysia (USM), Penang, Malaysia

*For correspondence: Email: abuatheer1@yahoo.com; Tel: +971-2-5011317, +971-552987641/507528850; Fax: +971-2-5011682

Received: 18 May 2013 Revised accepted: 20 March 2014

Tropical Journal of Pharmaceutical Research, May 2014; 13(5): 731-737

http://dx.doi.org/10.4314/tjpr.v13i5.12

Abstract

Purpose: To investigate the frequency of glucose-6-phosphate dehydrogenase (G6PD) variants and their associated enzyme deficiencies among different age groups of individuals in Abu Dhabi, United Arab Emirates (UAE).

Methods: A total of 15,995 patients (6302 UAE nationals and 9693 non-UAE nationals) who presented at Mafraq Hospital, Abu Dhabi, UAE between January 2006 and January 2009 were screened for G6PD deficiency using fluorescent spot test. Molecular analysis including polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP), denaturing high performance liquid chromatography (DHPLC) and DNA sequencing were utilized to identify common mutations in individuals with G6PD deficiency.

Results: The prevalence of G6PD deficiency among UAE nationals was 7.4% and non-UAE nationals 3.8%. UAE males showed prevalence of 11.6% while for UAE females it was 3.6%. The prevalence of G6PD deficiency among non-UAE nationals was 5 and 1.7% for males and females, respectively. The Mediterranean mutation, 563C→T, was predominant in non-UAE nationals.

Conclusion: G6PD Mediterranean mutation is the most prevalent mutation underlying G6PD deficiency followed by Aures mutations in both UAE nationals and non-UAE nationals. On the other hand, Africa A^- was found to be more in non-UAE compared with UAE nationals.

Keywords: Glucose-6-Phosphate Dehydrogenase deficiency, Mutation, Abu Dhabi, Polymerase chain reaction–restriction, Fragment length polymorphism