Abstract
Purpose: To determine the effects of
temperature, pressure and extraction time on oil yield
obtained from Polygonatum odoratum as well as the
optimum processing conditions for supercritical carbon
dioxide (CO2) extraction.
Methods: Supercritical CO2
extraction technology was adopted in this experiment to
study the process of extraction of volatile oil from
Polygonatum odoratum while gas chromatograph-mass
spectrometer (GC-MS) technique was employed to analyze
the chemical composition of the volatile oil. Response
surface methodology (RSM) was applied to optimize
supercritical CO2 process conditions.
Results: The determined optimal
conditions were as follows: extraction pressure, 27 MPa;
extraction temperature, 50 °C; and extraction time,
97.10 min. Under these conditions, the predicted yield
of Polygonatum volatile oil was 2.04 % as against an
actual oil yield of 2.02 % of the seeds. GC-MS analysis
showed that the extract obtained by supercritical CO2
extraction was richer in fatty acids and flavoring
substances than that obtained by hydrodistillation.
Conclusion: Using RSM can optimize
conditions of Polygonatum volatile oil. This provides a
scientific basis for further research and development of
healthy foods, as well as comprehensive utilization of
Polygonatum odoratum.
Keywords: Polygonatum odoratum,
Volatile oil, Supercritical fluid, Carbon dioxide,
Extraction, Gas chromatograph-mass spectrometer
