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Original Research Article
Cytotoxic Effect
of Ethanol Extract of Convolvulus arvensis L
(Convolvulaceae) on Lymphoblastic
Leukemia Jurkat Cells
Mohammad Saleem1,2*,
M Imran Qadir2, Bashir Ahmad1,
Uzma Saleem2, Faiza Naseer2,
Valerie Schini-Kerth3, Maqsood Ahmad2
and Khalid Hussain1
1University College
of Pharmacy, University of the Punjab, Lahore, 2College
of Pharmacy, GC University, Faisalabad, Pakistan,
3UMR CNRS 7213,
Laboratoire de Biophotonique et Pharmacologie,
UniversiteŽ de Strasbourg, FaculteŽ de Pharmacie,
Illkirch, France
*For
correspondence: Email:
saleem2978@hotmail.com
Received: 12 June 2013
Revised accepted: 22 March 2014
Tropical
Journal of Pharmaceutical Research, May 2014;
13(5): 705-709
http://dx.doi.org/10.4314/tjpr.v13i5.8
Abstract
Purpose: To evaluate the cytotoxic
effect of ethanol extract of aerial parts of Convolvulus
arvensis against lymphoblastic leukemia, Jurkat cells.
Methods:
The aerial parts of C.
arvensis were collected, identified, powdered and soaked
in ethanol. The extract was filtered and evaporated, and
the residue assessed for cytotoxic activity in Jurkat
cell line. The cells were exposed to different
concentrations (10, 25, 50, 75 and 100 ”g/mL) of the
extract to determine cell viability, cell
proliferation, apoptosis using Trypan blue exclusion
assay,
3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
(MTS) assay and fluorescent activated cell sorter (FACS)
analysis, respectively.
Results: Trypan blue exclusion assay
and MTS assay results indicate that the ethanol extract
decreased the number of living cells in a
concentration-dependent fashion. The results of FACS
analysis showed that the lowest concentration of the
extract (10 ”g/mL) was most effective for the induction
of apoptosis as it induced maximum apoptosis (85.34 %)
and the highest concentration (100 ”g/mL) was less
effective as it induced less apoptosis (53.70 %) in
Jurkat cells (p < 0.05).
Conclusion: The ethanol extract of C.
arvensis has significant cytotoxic activity against the
selected cancer cell line. Furthermore, apoptotic effect
was more prominent at lower doses and necrosis at higher
doses of the extract.
Keywords:
Convolvulus arvensis; (MTS)
3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
assay; Trypan blue exclusion assay, Apoptosis, Necrosis |
