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Original Research Article
Effect of Bisphenol A on
the Levels of Vitellogenin and Metallothionein in Adult
Male Carp, Cyprinus carpio carpio Linnaeus, 1758
Promy Virk, Amena Ali M
Al-Sakran and Mai A Elobeid*
Department of Zoology, King
Saud University, PO Box 22452,Riyadh-11495, Saudi Arabia
*For correspondence:
Email:
maielobeid@gmail.com; Tel:
966596404543
Received: 30 January 2014
Revised accepted: 2 June
2014
Tropical
Journal of Pharmaceutical Research, July 2014;
13(7): 1107-1112
http://dx.doi.org/10.4314/tjpr.v13i7.14
Abstract
Purpose: To
investigate the potential role of bisphenol A (BPA) in
inducing endocrine disruption in fish.
Methods:
Adult male koi carps, Cyprinus carpio carpio (n = 60,
mean body weight = 125 ± 12.288 g and mean length = 24 ±
1.392 cm) were exposed to three graded concentrations of
BPA (10, 100 and 1000 µg/L) for a period of 21 days. A
single dose of 17-β estradiol (1 ng/l) was used as
positive control.
Results: The
mean plasma concentration of vitellogenin (VTG)
significantly increased in the carps exposed to 100 µg
BPA/L reaching a mean value of 14600.0 ± 1400.0 ng/ml in
comparison to 1140.00 ± 34.64 ng/ml observed for the
control group,. However, BPA at all experimental
concentrations had no significant effect on
metallothionein levels in the liver, but the levels in
the kidney were significantly decreased in all BPA-exposed
groups in comparison to the level recorded for control
(181.69 ± 0.77 pg/g wet tissue).
Conclusion: Exposure to BPA modulates plasma
vitellogenin at median concentration and renal
metallothionein levels at all concentrations in carp.
Thus, these parameters are potential biomarkers in the
biomonitoring of the aquatic environment for estrogenic
chemicals.
Keywords: Carp, Bisphenol A, Vitellogenin,
Metallothionein, Endocrine disruptors |
