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Original Research Article
In vitro Evaluation of
PEGylated-Mucin Matrix as Carrier for Oral Delivery of
Metformin Hydrochloride
MA Momoh1*, MO
Adedokun2, MU Adikwu1 and CE
Ibezim1
1Drug Delivery Research
Unit, Department of Pharmaceutics, University of
Nigeria, Nsukka 410001, 2Department of Pharm.
Tech and Pharmaceutical Microbiology, University of Uyo,
Uyo, Nigeria
*For correspondence:
Email:
jointmomoh@yahoo.com; Tel:
234-8037784357
Received: 2 October 2013
Revised accepted: 16 March
2014
Tropical
Journal of Pharmaceutical Research, July 2014;
13(7): 1039-1045
http://dx.doi.org/10.4314/tjpr.v13i7.5
Abstract
Purpose: To
formulate metformin hydrochloride-loaded PEGylated-mucin
microparticles and evaluate their in vitro properties.
Method:
Three different formulations of metformin hydrochloride
(MTH) (PEG-M1, PEG-M2 and PEG-M3) were prepared using
PEGylation method. PEG-8000 and snail mucin, in a ratio
of 1:3, were PEGylated together using solvent
interaction principle. Loading of MTH into the matrix
was by diffusion method and the microparticles
characterized for particle size, zeta potential,
polydispersity index, stability and in vitro release in
phosphate buffer (pH 7.4).
Results:
Maximum yield and encapsulation were 97 and 87 %
respectively. Zeta potential was -37.7, - 42.3 and -46.2
mV for PEG-M1, PEG-M2 and PEG-M3 with a corresponding
polydispersity index (PDI) of 0.320, 0.374 and 0.398,
respectively. Particles size was 85, 115, and 145 µm for
PEG-M1, PEG-M2 and PEG-M3, respectively, and they showed
a unimodal distribution. Drug release was biphasic and
exhibited controlled release pattern with maximum
release of 92 % in 18 h compared to 81 % in 6 h for the
conventional formulation.
Conclusion: Extended release metformin hydrochloride
formulations were successfully developed using PEGylated
mucin matrices.
Keywords: Drug delivery, Extended release,
Polyethylene glycol, Mucin PEGylation, Encapsulation,
Zeta potential, Polydispersity index |
