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Original Research Article
Larvicidal Activity of
Isodon japonicus var. glaucocalyx (Maxim.)
H.W.Li Essential Oil to Aedes aegypti L. and its
Chemical Composition
Xin Chao Liu1,
Qi Yong Liu2, Ligang Zhou3 and Zhi
Long Liu1*
1Department of Entomology,
China Agricultural University, 2 Yuanmingyuan West Road,
Haidian District, Beijing 100193, 2State Key
Laboratory for Infectious Disease Prevention and
Control, National Institute for Communicable Disease
Control and Prevention, Chinese Center for Disease
Control and Prevention, Beijing, 102206, 3Department
of Plant Pathology, China Agricultural University, 2
Yuanmingyuan West Road, Haidian District, Beijing,
100193, China
*For correspondence:
Email:
zhilongliu@cau.edu.cn; Tel:
+86-10-62732800; Fax: +86-10-62732800
Received: 29 January 2014
Revised accepted: 24 July
2014
Tropical
Journal of Pharmaceutical Research, September 2014;
13(9): 1471-14 76
http://dx.doi.org/10.4314/tjpr.v13i9.13
Abstract
Purpose: To determine the larvicidal
activity of the essential oil derived from Isodon
japonicus var. glaucocalyx (Maxim.) H.W.Li (Labiatae)
aerial parts at flowering stage against the larvae of
Aedes aegypti L.
Methods: The essential oil of I.
japonicus var. glaucocalyx aerial parts was obtained by
hydrodistillation and analyzed by gas chromatography
(GC) and gas chromaotography-mas spectrometry (GC-MS).
The activity of the essential oil was evaluated, using
World Health Organization (WHO) procedures, against the
fourth larvae of A. aegypti for 24 h, and larval
mortality recorded at various essential oil
concentrations ranging from 12.5 - 200 μg/mL.
Results: A total of 34 components of
the essential oil of I. japonicus var. glaucocalyx were
identified. The principal compounds of the essential oil
were thujone (9.65 %), morillol (8.14 %), caryophyllene
oxide (7.68 %), β-caryophyllene (7.60 %), α-terpineol
(7.22 %), 1,8-cineole (7.09 %), linalool (5.56 %), Z-caryophyllene
(5.10 %) and γ-eudesmol (4.71 %). The essential oil
exhibited larvicidal activity against A. aegypti with a
median lethal concentration (LC50) of 40.82
μg/mL.
Conclusion: The findings indicate that
the essential oil of I. japonicus var. glaucocalyx
aerial parts has potentials for use in the control of A.
aegypti larvae and may be useful in the search for
newer, safer and more effective natural compounds for
use as larvicides.
Keywords: Isodon japonicus var.
glaucocalyx, Aedes aegypti, Larvicidal activity,
Mosquito, Essential oil |
