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Original Research Article
Evaluation of the
Antioxidant Activities of Ya-hom Intajak, a Thai Herbal
Formulation, and its Component Plants
Jantanarak Tuekaew1,
Nisarat Siriwatanametanon1, Yuvadee
Wongkrajang2, Rungravi Temsiririrkkul1
and Ibrahim Jantan3*
1Department of Pharmaceutical
Botany, 2Department of Physiology, Faculty of
Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road,
Rajathevi, Bangkok, 10400, Thailand, 3Drug
and Herbal Research Centre, Faculty of Pharmacy,
Universiti Kebangsaan, Jalan Raja Muda Abdul Aziz, 50300
Kuala Lumpur, Malaysia
*For correspondence:
Email:
profibj@gmail.com; Tel: +603
92897315; Fax: +60326983271
Received: 25 February 2014
Revised accepted: 3 August
2014
Tropical
Journal of Pharmaceutical Research, September 2014;
13(9): 1477-1485
http://dx.doi.org/10.4314/tjpr.v13i9.14
Abstract
Purpose: To evaluate the antioxidant
effect of 80 % ethanol extract and its n-hexane and
dichloromethane fractions of Ya-hom Intajak, and its 47
medicinal plants components.
Methods: The 80 % ethanol extract
and its n-hexane and dichloromethane fractions were
investigated on human low-density lipoprotein (LDL)
peroxidation by thiobarbituric acid reactive substances
(TBARS) assay. Antioxidant effect was also determined by
2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging
capacity and ferric reducing antioxidant power (FRAP)
assays.
Results: The extract of Ya-hom
Intajak exhibited moderate DPPH scavenging activity with
an IC50 value of 99.08 μg/ml and a FRAP value
of 1.12 mmol FeSO4/g. Among the individual plants,
Terminalia chebula Retz., Caesalpinia sappan L.,
Cinnamomum bejolghota (Buch.-Ham.) and Ci. verum J.
Presl, showed strong antioxidant activities. T. chebula
was the most potent plant in the DPPH assay while Ca.
sappan had the highest FRAP value. Among the extracts,
the dichloromethane fraction showed the highest
inhibitory effect on LDL peroxidation with a half
maximal inhibitory concentration (IC50) value
comparable to that of probucol (0.82 μg/ml). Pearson
correlation analysis revealed that total phenolic
content (TPC) showed high positive correlations with
FRAP (r = 0.908, p < 0.01) and DPPH activities (r =
0.648, p < 0.01).
Conclusion: Ya-hom Intajak is a
valuable source of natural antioxidants and has a
potential application as a cardiovascular protective
formulation.
Keywords: Ya-hom Intajak, Antioxidant
activity, Terminalia chebula Retz., Caesalpinia sappan
L., Cinnamomum bejolghota (Buch.-Ham.), Ci. verum J.
Presl , Phenolic content |
