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Original Research Article
Antioxidant Capacity,
Radical Scavenging Kinetics and Phenolic Profile of
Methanol Extracts of Wild Plants of Southern Sonora,
Mexico
EF Moran-Palacio1,
LA Zamora-Álvarez1, NA Stephens-Camacho2,
GA Yáñez-Farías1, A Virgen-Ortiz1,
O Martínez-Cruz3 and JA Rosas-Rodríguez1*
1Departamento de Ciencias
Químico Biológicas y Agropecuarias, Universidad de
Sonora Unidad Regional Sur. Navojoa, Sonora, Apartado
Postal 85390, 2Departamento de Nutrición
Humana. Universidad Estatal de Sonora Unidad Académica
Navojoa. Navojoa, Sonora, 3Departamento de
Investigación y Posgrado en Alimentos, Universidad de
Sonora. Blvd. Luis Encinas y Rosales s/n Col. Centro,
Apartado Postal 1658, C.P. 83000, Hermosillo, Sonora,
Mexico
*For correspondence:
Email:
jrosas@navojoa.uson.mx; Tel:
01(642)4259950 Ext 7057, 01(642)4259952 Ext 7057
Received: 3 December 2013
Revised accepted: 16 August
2014
Tropical
Journal of Pharmaceutical Research, September 2014;
13(9): 1487-1493
http://dx.doi.org/10.4314/tjpr.v13i9.15
Abstract
Purpose: To investigate the
antioxidant and phenolic profiles of methanol extracts
from Rhizophora mangle L, Krameria erecta, Lycium
berlandieri Dunal, Vallesia glabra Link and Forchammeria
watsonii Rose.
Methods: The free radical 2,
2-diphenyl-1-picrylhydrazyl (DPPH) method was used for
antioxidant assay of methanol plant extracts. Phenolic
compounds determination was carried out by high
performance liquid cromatography (HPLC) using an
analytical C18 column eluting with acetonitrile on a
gradient program.
Results: Extract from Rhizophora
mangle L and Krameria erecta displayed 5-fold higher
antioxidant capacity than ascorbic acid with DPPH
reduction values of 95.71 and 91 %, respectively.
Anti-radical kinetic analysis and stoichiometric
constant values suggest that the compounds responsible
for antioxidant activity are polyphenols and flavonoids
with a free or mono-substituted catechol group. Ferulic
and vanillic acids had higher contents in Rhizophora
mangle L (688.45 ± 18.21 and 7.13 ± 0.125 mg/g,
respectively) and Krameria erecta (619.92 ± 28.16 mg/g
and 22.29 ± 0.72 mg/g, respectively) than the other
constituents.
Conclusion: Rhizophora mangle and
Krameria erecta possess five times the antioxidant
activity of ascorbic acid and also demonstrate high
phenolic contents, which supports the beneficial
properties attributed to these plants in traditional
medicine, and also justifies the use of the plants as
alternative medicines.
Keywords: Rhizophora mangle, Krameria
erecta, Ferulic acid, vanillic acid, Antioxidant
capacity, Alternative medicines, Phenolics, Anti-radical
kinetics |
