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Original Research Article
Determination and
Distribution Study of Pogostone in Rat Tissues by
Ultra-Fast Liquid Chromatography
Hai-ming Chen1,2,
Lan Wang2, Xiao-li Wu1,3, Chu-wen
Li1,4, You-liang Xie1, Yu-hong Liu1,
Yong-zhuo Liang1, Xiao-ying Chen1,
Xiao-ping Lai1,5, Jian-nan Chen1,5,
Yu-cui Li 1* and, Zi-ren Su1,5*
1College of Chinese Medicines,
Guangzhou University of Chinese Medicine, Guangzhou
510006, 2The First Affiliated Hospital of
Chinese Medicine, Guangzhou University of Chinese
Medicine, Guangzhou 510405, 3Faculty of
Health Sciences, University of Macau, Macau 999078,
4State Key Laboratory of
Quality Research in Chinese Medicine, Institute of
Chinese Medical Sciences, University of Macau, Macau
999078,
5Dongguan Mathematical
Engineering Academy of Chinese Medicine, Guangzhou
University of Chinese Medicine, Dongguan 523808, China
*For correspondence:
Email:
liyucui@gzucm.edu.cn,
suziren@gzucm.edu.cn; Tel:
+86 20 39358517; Fax: +86 20 3935 8390
Received: 11 October 2014
Revised accepted: 12 January 2015
Tropical Journal of Pharmaceutical Research,
February 2015;
14(2):
279-286
http://dx.doi.org/10.4314/tjpr.v14i2.13
Abstract
Purpose: To develop and validate a
rapid, sensitive and reliable ultra-fast liquid
chromatography (UFLC) method with photodiode array (PDA)
detection for the determination of pogostone (PO) in rat
tissues using honokiol as internal standard (IS).
Methods: Rats were randomly divided
into two groups (intravenous administration group and
oral administration group) and given of a single dose of
10 mg/kg PO by intravenous administration and oral
administration, respectively. After intravenous
injection, the rats were sacrificed at 15, 60 and 360
min, while rats, after oral administration, were
euthanasized at 30, 90 and 360 min, respectively. For
the analysis of the preparation, optimal chromatographic
conditions were determined using Acquity UPLC BEH C18
column with acetonitrile-water containing 0.1 % formic
acid (55:45, v/v) as the mobile phase, at a flow rate of
400 µL/min. UV detection wavelength was set at 310 nm
with temperature maintained at 30 °C.
Results: Good linear relationship of
calibration curve (r > 0.9984) was achieved over the
range of 0.1 - 40 μg/mL for all the tissue samples. The
limit of quantification (LOQ) and limit of detection (LOD)
were 0.1 and 0.05 μg/mL, respectively. This method
proved to have good precision, accuracy, stability,
extraction recovery and matrix effect for tissue
distribution studies of PO in rats.
Conclusion: The developed method is
suitable for tissue distribution studies in rats
following intravenous and oral administration of PO at a
dose of 10 mg/kg.
Keywords: Ultra-fast liquid
chromatography, Tissue distribution, Pogostone, Honokiol,
Rats |
