of the Leaves of Endemic Stachys pseudopinardii
G Dulger* and C Aki
Department of Biology,
Faculty of Science and Arts, Canakkale Onsekiz Mart
University, 17100 Canakkale, Turkey
author: E-mail: firstname.lastname@example.org
Received: 20 February
Revised accepted: 25 April 2009
Journal of Pharmaceutical Research, August 2009; 8(4):
extract of the leaves of Stachys pseudopinardii R.
Bhattacharjee and Hub.–Mor. (Lamiaceae) were
investigated for their antimicrobial activities.
The antimicrobial activity of the leaf extract of the
plant was tested against Bacillus subtilis ATCC 6633,
Bacillus cereus ATCC 7064, Staphylococcus aureus ATCC
6538P, Escherichia coli ATCC 10538, Proteus vulgaris
ATCC 6899, Salmonella typhimurium CCM 5445 and
Pseudomonas aeruginosa ATCC 27853, as well as Candida
albicans ATCC 10239, Debaryomyces hansenii DSM 70238,
Kluyveromyces fragilis ATCC 8608 and Rhodotorula rubra
DSM 70403, by disc diffusion and microdilution methods.
Selected antibacterial agents (penicillin, tobramycin
and ampicillin) and antifungal agents (nystatin,
clotrimazole and ketoconazole) antibiotics were used as
positive reference standards in the tests.
The extracts showed strong antibacterial activity
against Bacillus cereus ATCC 7064, with an inhibition
zone of 25.0 mm, and minimum inhibitory concentration
(MIC) and minimum bactericidal concentration (MBC) of 16
and 32 µg/mL, respectively. Debaryomyces hansenii DSM
70238 was among the most susceptible of the yeast
cultures, with an inhibition zone of 17.0 mm and MIC and
minimum fungicidal concentration (MFC) of 32 and 32 µg/mL,
respectively. The extract exhibited moderate activity
against the other test microorganisms.
The results demonstrate that the ethanol extract of the
leaves of Stachys pseudopinardii has significant
antimicrobial activity and suggest that it may be useful
in the treatment of infections.
Stachys pseudopinardii, ethanol extract, antimicrobial
activity, MIC, MBC, MFC.